AbFlex Bcl6 antibody (rAb)
Host / Isotype
Mouse / IgG2a
Reactivity
Human
Applications
WB
Cat No : 91287,91288 91287
Synonyms
验证数据展示
产品信息
| Tested Applications |
WB
Applications Validated by Active Motif: WB: 0.5 – 2 ug/ml AbFlex recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones. For details on the parental clone, see Catalog No. 61193. |
| Tested Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | This antibody was raised against full length human Bcl6 (B-cell lymphoma 6 protein) by genetic immunization. (Garcia et. al., 2006. Genetic Immunization: A New Monoclonal Antibody for the Detection of BCL-6 Protein in Paraffin Sections. J. Histochemistry & Cytochemistry, vol. 54(1): 31-38). |
| Full Name | AbFlex Bcl6 antibody (rAb) |
| Synonyms | BCL6, B-Cell CLL/Lymphoma 6, ZBTB27, Zinc Finger Protein 51, B-Cell Lymphoma 5 Protein, ZNF51, BCL6A, Protein LAZ-3, B-Cell Lymphoma 6 Protein, Zinc Finger And BTB Domain-Containing Protein 27, B-Cell Lymphoma 6 Protein Transcript, BCL-5, Cys-His2 Zinc Finger Transcription Factor, BCL-6 |
| Molecular weight | 80 kDa |
| GenBank accession number | NP_001124317 | RRID | AB_2793833 | Purification Method | Protein A Chromatography |
| Buffer | 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035% sodium azide, and 30% glycerol. Sodium azide is highly toxic. |
| Storage | Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage. |
背景介绍
AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis Tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex Bcl6 antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. Bcl6 (B-cell lymphoma 6 protein, Bcl5, LAZ3, ZBTB27, ZNF51) is sequence-specific transcriptional repressor that is required for the formation of lymph node germinal centers (GC). Bcl6 is a member of the BTB-POZ family of transcriptional regulators and contains six Kruppel-type zinc fingers. The POZ domain in the amino terminus of Bcl6 is involved in the recruitment of corepressor proteins including NCoR, BCoR, SMRT, Mi-2/NURD and several HDACs. Bcl6 promotes proliferative expansion of B-cells in the germinal center through repression of genes regulating cell cycle and differentiation. Bcl6 also suppresses the DNA damage response, to prevent class-switch recombination being repaired. Unregulated expression of Bcl6 is involved in the development of many B-cell lymphomas.