• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

AbFlex Histone H3, C-terminal antibody (rAb, Rabbit)

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

ChIP, WB

Cat No : 91299,91300 91299

Synonyms

AbFlex, recombinant antibody, Histone H3, H3 c-terminal, antibody,

验证数据展示

  • AbFlex Histone H3 antibody (rAb) tested by ChIP Chromatin immunoprecipitation (ChIP) was performed with 30 ug of chromatin from Farage human lymph-node derived B cells and 4.5 ug AbFlex Histone H3 antibody. ChIP DNA was used in qPCR with the gene-specific primers as indicated. Data are presented as Binding Events Detected per 1000 Cells using Active Motif's Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.

    AbFlex Histone H3 antibody (rAb) tested by ChIP Chromatin immunoprecipitation (ChIP) was performed with 30 ug of chromatin from Farage human lymph-node derived B cells and 4.5 ug AbFlex Histone H3 antibody. ChIP DNA was used in qPCR with the gene-specific primers as indicated. Data are presented as Binding Events Detected per 1000 Cells using Active Motif's Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.

  • AbFlex Histone H3, C-term antibody tested by Western blot. 20 ug of HeLa nuclear extract* was run on SDS-PAGE and probed with AbFlex Histone H3, C-term antibody at 0.1 ug/ml.

    AbFlex Histone H3, C-term antibody tested by Western blot. 20 ug of HeLa nuclear extract* was run on SDS-PAGE and probed with AbFlex Histone H3, C-term antibody at 0.1 ug/ml.

产品信息

Tested Applications ChIP, WB

Applications Validated by Active Motif: ChIP: 4 - 5 ug per ChIP WB*: 0.01 – 0.5 ug/ml *Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot.
Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen This antibody was raised against a peptide at the C-terminus of human Histone H3.
Full Name AbFlex Histone H3, C-terminal antibody (rAb, Rabbit)
Synonyms AbFlex, recombinant antibody, Histone H3, H3 c-terminal, antibody,
Molecular weight 17 kDa
GenBank accession numberNP_003522
Purification Method Protein A Chromatography
Buffer Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035% sodium azide, 30% glycerol. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

背景介绍

AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis Tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex Histone H3, C-term was expressed as full-length IgG with rabbit immunoglobulin heavy and light chains in CHO cells. Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4).