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AbFlex c-Myc antibody (rAb)

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB

Cat No : 91213,91214 91213

Synonyms

验证数据展示

  • AbFlex c-Myc antibody (rAb) tested by Western Blot. HEK293 cells were transiently transfected with empty vector (Lane 1) or with human c-Myc protein (Lane 2). 10 ug HEK293 whole cell extract was loaded on SDS-PAGE gel and probed with AbFlex c-Myc antibody at 1 ug/ml. MW: 68 kDa.

    AbFlex c-Myc antibody (rAb) tested by Western Blot. HEK293 cells were transiently transfected with empty vector (Lane 1) or with human c-Myc protein (Lane 2). 10 ug HEK293 whole cell extract was loaded on SDS-PAGE gel and probed with AbFlex c-Myc antibody at 1 ug/ml. MW: 68 kDa.

产品信息

Tested Applications WB

Applications Validated by Active Motif: WB: 1 ug/ml dilution AbFlex recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones. For details on the parental clone, see Catalog No. 61075.
Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Immunogen This antibody was raised against amino acids 1-262 of human c-Myc (myc proto-oncogene protein).
Full Name AbFlex c-Myc antibody (rAb)
Synonyms
Molecular weight 68 kDa
GenBank accession numberNP_002458
RRIDAB_2793806
Purification Method Protein A Chromatography
Buffer Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035% sodium azide, 30% glycerol. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

背景介绍

AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis Tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. c-Myc is a sequence specific DNA binding transcription factor that regulates gene expression, especially genes involved in cell division. Myc family members contain a basic Helix-Loop-Helix leucine zipper (bHLHZ) domain. Myc family proteins associated with thousands of genes, especially those involved in cell growth and proliferation. Myc serves as an integrator of signal transduction, linking extracellular signals to an acute cellular growth response. Mutations and overexpression of c-Myc are associated with cell proliferation and cancer.