• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Acetyl-Lysine antibody (rAb) (Clone RM101)

Host / Isotype

Rabbit / IgG

Reactivity

Human, Not Species Specific

Applications

ICC, WB

CloneNo.

RM101

Cat No : 91315 91315

Synonyms

Acetyl-Lysine, Lysine N-acetylation, acetylation, recombinant antibody, Histone Acetyltransferases, HATs, Clone RM101

验证数据展示

  • Western blot of Acetyl-Lysine antibody (rAb). HeLa cells nontreated or treated with Trichostatin A (TSA), were probed using Acetyl-Lysine antibody (RM101) at 0.5 ug/ml.

    Western blot of Acetyl-Lysine antibody (rAb). HeLa cells nontreated or treated with Trichostatin A (TSA), were probed using Acetyl-Lysine antibody (RM101) at 0.5 ug/ml.

  • Immunofluorescence stain of Acetyl-Lysine antibody (rAb). HeLa cells were stained using Acetyl-Lysine antibody (RM101) at 2 ug/ml (red). Actin filaments was labeled with fluorescein phalloidin (green), and nucleus stained with DAPI (blue).

    Immunofluorescence stain of Acetyl-Lysine antibody (rAb). HeLa cells were stained using Acetyl-Lysine antibody (RM101) at 2 ug/ml (red). Actin filaments was labeled with fluorescein phalloidin (green), and nucleus stained with DAPI (blue).

产品信息

Tested Applications ICC, WB

Applications Validated by Active Motif: WB: 0.5 - 2 ug/ml ICC: 2 - 10 ug/ml
Tested Reactivity Human, Not Species Specific
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Acetyllysine-BSA
Full Name Acetyl-Lysine antibody (rAb) (Clone RM101)
Synonyms Acetyl-Lysine, Lysine N-acetylation, acetylation, recombinant antibody, Histone Acetyltransferases, HATs, Clone RM101
Molecular weight
GenBank accession numberN/A
Purification Method Protein A Chromatography
Buffer PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

背景介绍

Lysine N-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. Acetylation of histones can occur at several different lysine positions in the histone tail, and is performed by Histone Acetyltransferases (HATs) such as HAT1 or GCN5. Acetylation of histones is often associated with transcriptional activation. Clone RM101 reacts to lysine-acetylated proteins. No cross reactivity with nonacetylated lysine, and lysines with other modifications.