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Species Reactivity
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验证数据展示

  • FC experiment of SH-SY5Y using 20332-1-AP (same clone as 20332-1-PBS)

    FC experiment of SH-SY5Y using 20332-1-AP (same clone as 20332-1-PBS)

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug Adenosine A1 Receptor Polyclonal antibody (20332-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 20332-1-PBS in a different storage buffer formulation.

  • WB analysis of mouse eye using 20332-1-AP (same clone as 20332-1-PBS)

    WB analysis of mouse eye using 20332-1-AP (same clone as 20332-1-PBS)

    mouse eye tissue were subjected to SDS PAGE followed by western blot with 20332-1-AP (Adenosine A1 Receptor antibody at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20332-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 20332-1-AP (same clone as 20332-1-PBS)

    IHC staining of mouse brain using 20332-1-AP (same clone as 20332-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20332-1-AP (Adenosine A1 Receptor antibody at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20332-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 20332-1-AP (same clone as 20332-1-PBS)

    IHC staining of mouse brain using 20332-1-AP (same clone as 20332-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20332-1-AP (Adenosine A1 Receptor antibody at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20332-1-PBS in a different storage buffer formulation.

Adenosine A1 Receptor Polyclonal antibody, PBS Only

Adenosine A1 Receptor Polyclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA
Cat No. 20332-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, FC (Intra), Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

20332-1-PBS targets Adenosine A1 Receptor in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 Adenosine A1 Receptor fusion protein Ag14092 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 adenosine A1 receptor
别名
计算分子量 326 aa, 37 kDa
观测分子量 39 kDa
GenBank蛋白编号BC026340
基因名称 Adenosine A1 Receptor
Gene ID (NCBI) 134
RRIDAB_2878673
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP30542
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ADORA1, also named as RDC7, is a receptor for adenosine. It is mediated by G proteins which inhibit adenylyl cyclase. Adenosine is an important mediator of ethanol intoxication and exerts some of its effects via ADORA1 in the central nervous system.