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Adiponectin Monoclonal antibody, PBS Only

Adiponectin Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5D8A7

Cat no : 66239-1-PBS

Print datasheet

Synonyms

ACDC, ACRP30, adiponectin, ADIPOQ, ADIPQTL1, ADPN, APM 1, APM1, GBP28, Gelatin binding protein

验证数据展示

  • IHC staining of mouse skeletal muscle using 66239-1-Ig (same clone as 66239-1-PBS)

    IHC staining of mouse skeletal muscle using 66239-1-Ig (same clone as 66239-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 66239-1-Ig (Adiponectin antibody) at dilution of 1:32000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66239-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Adiponectin antibody included
  • IHC staining of mouse skeletal muscle using 66239-1-Ig (same clone as 66239-1-PBS)

    IHC staining of mouse skeletal muscle using 66239-1-Ig (same clone as 66239-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 66239-1-Ig (Adiponectin antibody) at dilution of 1:32000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66239-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Adiponectin antibody included
  • WB analysis of human adipose using 66239-1-Ig (same clone as 66239-1-PBS)

    WB analysis of human adipose using 66239-1-Ig (same clone as 66239-1-PBS)

    human adipose tissue were subjected to SDS PAGE followed by western blot with 66239-1-Ig (ADIPOQ Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66239-1-PBS in a different storage buffer formulation.

  • IF Staining of 3T3-L1 using 66239-1-Ig (same clone as 66239-1-PBS)

    IF Staining of 3T3-L1 using 66239-1-Ig (same clone as 66239-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed 3T3-L1 cells using Adiponectin antibody (66239-1-Ig, Clone: 5D8A7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 66239-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy Adiponectin Ready-To-Use IHC Kit. Adiponectin primary antibody included.

产品信息

66239-1-PBS targets Adiponectin in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Adiponectin fusion protein Ag17383 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name adiponectin, C1Q and collagen domain containing
Synonyms ACDC, ACRP30, adiponectin, ADIPOQ, ADIPQTL1, ADPN, APM 1, APM1, GBP28, Gelatin binding protein
Calculated Molecular Weight 244 aa, 26 kDa
Observed Molecular Weight 29 kDa
GenBank Accession NumberBC096308
Gene Symbol Adiponectin
Gene ID (NCBI) 9370
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ15848
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Adiponectin (AdipoQ), an adipocyte-derived hormone, is one of the most abundant adipokines in the blood circulation. Adiponectin modulates a number of metabolic processes, including improving INS sensitivity and anti-inflammatory activity. The role of AdipoQ in reproduction is not yet fully understood, but the expression of AdipoQ in reproductive tissues has been observed in various animals and humans, including chicken testis, bovine ovary, and human placenta. Adiponectin exerts its effects by activating a range of different signaling molecules via binding to two transmembrane AdipoQ receptors, AdipoR1 and AdipoR2. AdipoR1 is expressed primarily in the skeletal muscle, whereas AdipoR2 is predominantly expressed in the liver. AdipoQ May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors.