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验证数据展示

  • WB analysis of rat adipose using 83525-2-RR (same clone as 83525-2-PBS)

    WB analysis of rat adipose using 83525-2-RR (same clone as 83525-2-PBS)

    rat adipose tissue were subjected to SDS PAGE followed by western blot with 83525-2-RR (Adiponectin antibody) at dilution of 1:7500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83525-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00522-1

    Cytometric bead array standard curve of MP00522-1

    Cytometric bead array standard curve of MP00522-1, RAT Adiponectin Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83525-3-PBS. Detection antibody: 83525-2-PBS. Standard: Eg0872. Range: 0.313-40 ng/mL.

  • Cytometric bead array standard curve of MP00522-2

    Cytometric bead array standard curve of MP00522-2

    Cytometric bead array standard curve of MP00522-2, RAT Adiponectin Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83525-1-PBS. Detection antibody: 83525-2-PBS. Standard: Eg0872. Range: 0.313-40 ng/mL.

  • Affinity and Kinetic Characterization of 83525-2-RR

    Affinity and Kinetic Characterization of 83525-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83525-2-RR against Rat Adiponectin were performed. The affinity constant is 0.149 nM.

Rat Adiponectin Recombinant antibody, PBS Only (Detector)

Adiponectin Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA
Cat No. 83525-2-PBS

产品说明书

CloneNo. 240392D6

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, Cytometric bead array, Indirect ELISA

别名

Adipoq, Acrp30, Acdc, 240392D6

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83525-2-PBS targets Adiponectin as part of a matched antibody pair:

MP00522-1: 83525-3-PBS capture and 83525-2-PBS detection (validated in Cytometric bead array)

MP00522-2: 83525-1-PBS capture and 83525-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 mouse, rat
免疫原 Fusion Protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 adiponectin, C1Q and collagen domain containing
别名 Adipoq, Acrp30, Acdc, 240392D6
计算分子量26 kDa
观测分子量26-28 kDa
GenBank蛋白编号NM_144744.3
基因名称 Adiponectin
Gene ID (NCBI) 246253
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ8K3R4
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Adiponectin (AdipoQ), an adipocyte-derived hormone, is one of the most abundant adipokines in the blood circulation. Adiponectin modulates a number of metabolic processes, including improving INS sensitivity and anti-inflammatory activity. The role of AdipoQ in reproduction is not yet fully understood, but the expression of AdipoQ in reproductive tissues has been observed in various animals and humans, including chicken testis, bovine ovary, and human placenta. Adiponectin exerts its effects by activating a range of different signaling molecules via binding to two transmembrane AdipoQ receptors, AdipoR1 and AdipoR2. AdipoR1 is expressed primarily in the skeletal muscle, whereas AdipoR2 is predominantly expressed in the liver. AdipoQ May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors.