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验证数据展示

  • IHC staining of human liver using 25213-1-AP

    IHC staining of human liver using 25213-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25213-1-AP (Adropin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver using 25213-1-AP

    IHC staining of human liver using 25213-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25213-1-AP (Adropin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of SH-SY5Y using 25213-1-AP

    IF Staining of SH-SY5Y using 25213-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using Adropin antibody (25213-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Adropin Polyclonal antibody

Adropin Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 25213-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF/ICC, ELISA

别名

Adropin, C9orf165, energy homeostasis associated, ENHO, UNQ470

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25213-1-AP targets Adropin in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 Adropin fusion protein Ag19526 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 energy homeostasis associated
别名 Adropin, C9orf165, energy homeostasis associated, ENHO, UNQ470
计算分子量 75 aa, 8 kDa
GenBank蛋白编号BC022101
基因名称 Adropin
Gene ID (NCBI) 375704
RRIDAB_3085776
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6UWT2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Adropin is a nutritionally regulated peptide hormone that is primarily secreted by the liver and is important in maintaining metabolic and non-metabolic homeostasis. Adropin regulates glucose and fatty acid metabolism and exerts a potential protective effect on endothelial function through modulation of endothelial nitric oxide (NO) synthesis. It is associated with obesity and is involved in the prevention of insulin resistance, dyslipidemia, and impaired glucose tolerance (PMID: 22318315).

实验方案

Product Specific Protocols
IHC protocol for Adropin antibody 25213-1-APDownload protocol
IF protocol for Adropin antibody 25213-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols