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验证数据展示

  • WB analysis using 28686-1-AP

    WB analysis using 28686-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28686-1-AP (Ajuba antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse skin using 28686-1-AP

    IHC staining of mouse skin using 28686-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 28686-1-AP (Ajuba antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with AJUBA antibody included
  • IHC staining of mouse skin using 28686-1-AP

    IHC staining of mouse skin using 28686-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 28686-1-AP (Ajuba antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with AJUBA antibody included

Ajuba Polyclonal antibody

Ajuba Polyclonal Antibody for WB, IHC, ELISA
Cat No. 28686-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse, rat and More (1)

应用

WB, IHC, ELISA

别名

Ajuba, JUB, Protein ajuba

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells
Positive IHC detected inmouse skin tissue, human thyroid cancer tissue, mouse small intestine tissue, mouse testis tissue, rat skin tissue, rat small intestine tissue, rat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy AJUBA Ready-To-Use IHC Kit. AJUBA primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

28686-1-AP targets Ajuba in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 Human, mouse, rat
文献引用反应性human
免疫原 Ajuba fusion protein Ag29725 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 jub, ajuba homolog (Xenopus laevis)
别名 Ajuba, JUB, Protein ajuba
计算分子量 57 kDa
观测分子量 57 kDa
GenBank蛋白编号BC007580
基因名称 Ajuba
Gene ID (NCBI) 84962
RRIDAB_2881194
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96IF1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ajuba belongs to the LIM domain proteins, which are involved in the assembly of the extracellular matrix and, along with associated proteins, regulate target genes that connect the extracellular matrix and the cytoskeleton. Ajuba consists of three LIM domains at the C-terminal and is located in the cytoplasm where it interacts with a variety of other proteins. Ajuba contributes to the formation or strengthening of cell-cell adhesion and regulates cell growth and differentiation decisions. Ajuba participates in the assembly of the extracellular matrix. Ajuba also plays an important role in some diseases.

实验方案

Product Specific Protocols
WB protocol for Ajuba antibody 28686-1-APDownload protocol
IHC protocol for Ajuba antibody 28686-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Rep

ZNF451 favors triple-negative breast cancer progression by enhancing SLUG-mediated CCL5 transcriptional expression

Authors - Yu Zhang
View Article