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Alpha-1-microglobulin Recombinant antibody, PBS Only

Alpha-1-microglobulin Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240751G7

Cat no : 83678-3-PBS

Print datasheet

Synonyms

AMBP, Alpha 1-microglobulin, Alpha 1 microglycoprotein, Alpha 1 microglobulin, A1M

验证数据展示

  • WB analysis of human plasma using 83678-3-RR (same clone as 83678-3-PBS)

    WB analysis of human plasma using 83678-3-RR (same clone as 83678-3-PBS)

    human plasma were subjected to SDS PAGE followed by western blot with 83678-3-RR (Alpha-1-microglobulin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83678-3-PBS in a different storage buffer formulation.

  • WB analysis of human urine using 83678-3-RR (same clone as 83678-3-PBS)

    WB analysis of human urine using 83678-3-RR (same clone as 83678-3-PBS)

    human urine sample were subjected to SDS PAGE followed by western blot with 83678-3-RR (Alpha-1-microglobulin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83678-3-PBS in a different storage buffer formulation.

  • FC experiment of MCF-7 using 83678-3-RR (same clone as 83678-3-PBS)

    FC experiment of MCF-7 using 83678-3-RR (same clone as 83678-3-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug Alpha 1 microglobulin Recombinant antibody (83678-3-RR, Clone:240751G7) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83678-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83678-3-RR

    Affinity and Kinetic Characterization of 83678-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83678-3-RR against Human Alpha 1 microglobulin were performed. The affinity constant is 6.59 nM.

产品信息

83678-3-PBS targets Alpha-1-microglobulin in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name alpha-1-microglobulin/bikunin precursor
Synonyms AMBP, Alpha 1-microglobulin, Alpha 1 microglycoprotein, Alpha 1 microglobulin, A1M
Calculated Molecular Weight39kDa
Observed Molecular Weight30 kDa
GenBank Accession NumberNM_001633.4
Gene Symbol Alpha 1 microglobulin
Gene ID (NCBI) 259
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP02760
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Alpha 1 microglobulin, also known as HI30, is a 27-30 kDa glycoprotein, present in various body fluids. It belongs to the lipocalin superfamily of hydrophobic ligand-binding proteins forming an internal ligand-binding pocket. The protein acts as a mediator of bacterial adhesion to polymer surfaces and is involved in inhibiting renal lithogenesis.