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Alpha Actinin Monoclonal antibody, PBS Only

Alpha Actinin Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, rat, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E2E1

Cat no : 66895-1-PBS

Print datasheet

Synonyms

Alpha actinin 1, ACTN1, ACTN 1, actinin, alpha 1, actin

验证数据展示

  • WB analysis using 66895-1-Ig (same clone as 66895-1-PBS)

    WB analysis using 66895-1-Ig (same clone as 66895-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66895-1-Ig (a-Actinin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66895-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 66895-1-Ig (same clone as 66895-1-PBS)

    IHC staining of mouse heart using 66895-1-Ig (same clone as 66895-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66895-1-Ig (alpha Actinin antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66895-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Alpha Actinin antibody included
  • IHC staining of mouse heart using 66895-1-Ig (same clone as 66895-1-PBS)

    IHC staining of mouse heart using 66895-1-Ig (same clone as 66895-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66895-1-Ig (alpha Actinin antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66895-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Alpha Actinin antibody included
  • FC experiment of A431 using 66895-1-Ig (same clone as 66895-1-PBS)

    FC experiment of A431 using 66895-1-Ig (same clone as 66895-1-PBS)

    1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human Alpha Actinin (66895-1-Ig, Clone:1E2E1) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66895-1-PBS in a different storage buffer formulation.

  • IF Staining of C2C12 using 66895-1-Ig (same clone as 66895-1-PBS)

    IF Staining of C2C12 using 66895-1-Ig (same clone as 66895-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using Alpha Actinin antibody (66895-1-Ig, Clone: 1E2E1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66895-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy Alpha Actinin Ready-To-Use IHC Kit. Alpha Actinin primary antibody included.

产品信息

66895-1-PBS targets Alpha Actinin in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity Human, rat, mouse
Immunogen Alpha Actinin fusion protein Ag28383 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name actinin, alpha 1
Synonyms Alpha actinin 1, ACTN1, ACTN 1, actinin, alpha 1, actin
Calculated Molecular Weight 103 kDa
Observed Molecular Weight 100-105 kDa
GenBank Accession NumberBC015766
Gene Symbol Alpha Actinin
Gene ID (NCBI) 87
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP12814
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Alpha Actinins are highly conserved actin-binding proteins that form antiparallel homodimers with the actin-binding domains on each end of the molecule, allowing crosslinking of actin molecules. There are four alpha actinin isoforms identified: two ubiquitously expressed cytoskeletal isoforms (ACTN1 and ACTN4) and two striated muscle-specific sarcomeric isoforms (ACTN2 and ACTN3). ACTN1 is also alternatively spliced to form smooth muscle or brain specific isoforms. This antibody recognize endogenous ACTN1, while it may cross-react with ACTN2 and ACTN4.