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Alpha Actinin Monoclonal antibody, PBS Only

Alpha Actinin Monoclonal Antibody for WB,Indirect ELISA,IHC,IF,FC

Host / Isotype

Mouse / IgG2b

Reactivity

Human, rat, mouse

Applications

WB,Indirect ELISA,IHC,IF,FC

Conjugate

Unconjugated

CloneNo.

1E2E1

Cat No : 66895-1-PBS

Print datasheet

Synonyms

actinin, alpha 1, ACTN1, Alpha Actinin, Alpha actinin 1, F actin cross linking protein, FLJ40884, FLJ54432, Non muscle alpha actinin 1

验证数据展示

  • WB analysis using 66895-1-Ig (same clone as 66895-1-PBS)

    WB analysis using 66895-1-Ig (same clone as 66895-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66895-1-Ig (a-Actinin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66895-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 66895-1-Ig (same clone as 66895-1-PBS)

    IHC staining of mouse heart using 66895-1-Ig (same clone as 66895-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66895-1-Ig (alpha Actinin antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66895-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Alpha Actinin antibody included
  • IHC staining of mouse heart using 66895-1-Ig (same clone as 66895-1-PBS)

    IHC staining of mouse heart using 66895-1-Ig (same clone as 66895-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66895-1-Ig (alpha Actinin antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66895-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Alpha Actinin antibody included
  • FC experiment of A431 using 66895-1-Ig (same clone as 66895-1-PBS)

    FC experiment of A431 using 66895-1-Ig (same clone as 66895-1-PBS)

    1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human Alpha Actinin (66895-1-Ig, Clone:1E2E1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66895-1-PBS in a different storage buffer formulation.

  • IF Staining of C2C12 using 66895-1-Ig (same clone as 66895-1-PBS)

    IF Staining of C2C12 using 66895-1-Ig (same clone as 66895-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using Alpha Actinin antibody (66895-1-Ig, Clone: 1E2E1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66895-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy Alpha Actinin Ready-To-Use IHC Kit. Alpha Actinin primary antibody included.

产品信息

66895-1-PBS targets Alpha Actinin in WB, IF, FC, IHC, Indirect ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Applications WB,Indirect ELISA,IHC,IF,FC Application Description
Tested Reactivity Human, rat, mouse
Immunogen Alpha Actinin fusion protein Ag28383 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name actinin, alpha 1
Synonyms actinin, alpha 1, ACTN1, Alpha Actinin, Alpha actinin 1, F actin cross linking protein, FLJ40884, FLJ54432, Non muscle alpha actinin 1
Calculated Molecular Weight 103 kDa
Observed Molecular Weight 100-105 kDa
GenBank Accession NumberBC015766
Gene Symbol ACTN1
Gene ID (NCBI) 87
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP12814
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Alpha Actinins are highly conserved actin-binding proteins that form antiparallel homodimers with the actin-binding domains on each end of the molecule, allowing crosslinking of actin molecules. There are four alpha actinin isoforms identified: two ubiquitously expressed cytoskeletal isoforms (ACTN1 and ACTN4) and two striated muscle-specific sarcomeric isoforms (ACTN2 and ACTN3). ACTN1 is also alternatively spliced to form smooth muscle or brain specific isoforms. This antibody recognize endogenous ACTN1, while it may cross-react with ACTN2 and ACTN4.