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  • Featured Product
  • KD/KO Validated

Alpha Galactosidase A Monoclonal antibody, PBS Only

Alpha Galactosidase A Monoclonal Antibody for WB,Indirect ELISA,IHC,IF

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

2B2C5

Cat No : 66121-1-PBS

Print datasheet

Synonyms

Alpha D galactosidase A, Alpha galactosidase A, galactosidase, alpha

验证数据展示

  • WB analysis of HEK-293 using 66121-1-Ig (same clone as 66121-1-PBS)

    WB analysis of HEK-293 using 66121-1-Ig (same clone as 66121-1-PBS)

    WB result of GLA antibody (66121-1-Ig, 1:2000) with si-Control and si-GLA transfected HEK293 cells.. This data was developed using the same antibody clone with 66121-1-PBS in a different storage buffer formulation.

  • WB analysis using 66121-1-Ig (same clone as 66121-1-PBS)

    WB analysis using 66121-1-Ig (same clone as 66121-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66121-1-Ig (Alpha galactosidase A antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66121-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 66121-1-Ig (same clone as 66121-1-PBS)

    IHC staining of human kidney using 66121-1-Ig (same clone as 66121-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66121-1-Ig (Alpha galactosidase A antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66121-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 66121-1-Ig (same clone as 66121-1-PBS)

    IHC staining of human kidney using 66121-1-Ig (same clone as 66121-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66121-1-Ig (Alpha galactosidase A antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66121-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66121-1-Ig (same clone as 66121-1-PBS)

    IF Staining of HepG2 using 66121-1-Ig (same clone as 66121-1-PBS)

    Immunofluorescent analysis of HepG2 cells using 66121-1-Ig (Alpha galactosidase A antibody) at dilution of 1:50 and Rhodamine-Goat anti-Mouse IgG. This data was developed using the same antibody clone with 66121-1-PBS in a different storage buffer formulation.

产品信息

66121-1-PBS targets Alpha Galactosidase A in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB,Indirect ELISA,IHC,IF Application Description
Tested Reactivity human
Immunogen Alpha Galactosidase A fusion protein Ag7505 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name galactosidase, alpha
Synonyms Alpha D galactosidase A, Alpha galactosidase A, galactosidase, alpha
Calculated Molecular Weight 49 kDa
Observed Molecular Weight 49 kDa
GenBank Accession NumberBC002689
Gene Symbol GLA
Gene ID (NCBI) 2717
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP06280
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

GLA, also named as Melibiase and Alpha-galactosidase A, belongs to the glycosyl hydrolase 27 family. It hydrolyzes terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids. Fabry disease is an X-linked lysosomal storage disorder resulting from the deficient activity of GLA. Enzyme replacement therapy (ERT) with GLA is currently the most effective therapeutic strategy for patients with Fabry disease, a lysosomal storage disease.