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Amphiregulin Monoclonal antibody, PBS Only

Amphiregulin Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, pig

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A1G9

Cat no : 66433-1-PBS

Print datasheet

Synonyms

amphiregulin, AR, AREG, AREGB, CRDGF, SDGF

验证数据展示

  • WB analysis of A549 using 66433-1-Ig (same clone as 66433-1-PBS)

    WB analysis of A549 using 66433-1-Ig (same clone as 66433-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 66433-1-Ig (Amphiregulin antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66433-1-PBS in a different storage buffer formulation.

  • WB analysis using 66433-1-Ig (same clone as 66433-1-PBS)

    WB analysis using 66433-1-Ig (same clone as 66433-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66433-1-Ig (Amphiregulin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66433-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas cancer using 66433-1-Ig (same clone as 66433-1-PBS)

    IHC staining of human pancreas cancer using 66433-1-Ig (same clone as 66433-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66433-1-Ig (Amphiregulin antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66433-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AREG antibody included
  • IHC staining of human pancreas cancer using 66433-1-Ig (same clone as 66433-1-PBS)

    IHC staining of human pancreas cancer using 66433-1-Ig (same clone as 66433-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66433-1-Ig (Amphiregulin antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66433-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AREG antibody included
Planning an IHC experiment? We recommend our IHCeasy AREG Ready-To-Use IHC Kit. AREG primary antibody included.

产品信息

66433-1-PBS targets Amphiregulin in WB, IHC, Indirect ELISA applications and shows reactivity with human, rat, pig samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human, rat, pig
Immunogen Amphiregulin fusion protein Ag8907 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name amphiregulin
Synonyms amphiregulin, AR, AREG, AREGB, CRDGF, SDGF
Calculated Molecular Weight 252 aa, 28 kDa
Observed Molecular Weight 50 kDa, 37 kDa
GenBank Accession NumberBC009799
Gene Symbol Amphiregulin/AREG
Gene ID (NCBI) 374
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP15514
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Amphiregulin (AREG) is one of the ligands of the epidermal growth factor receptor (EGFR). AREG plays a central role in mammary gland development and branching morphogenesis in organs and is expressed both in physiological and in cancerous tissues. The AREG protein is synthesized as a 252-amino acid transmembrane precursor, pro-AREG. At the plasma membrane, pro-AREG is subjected to sequential proteolytic cleavages within its ectodomain and is then released as the soluble AREG protein. Depending on the cell type and microenvironment, AREG can be produced in multiple cellular and mature forms using alternative pro-AREG cleavage sites and glycosylation motifs. Post-translastional modfications of 50-kDa pro-AREG produces a major soluble 43-kDa form, 28-, 26-, 16-kDa membrane anchored forms, and soluble 21-, 19-, and 9-kDa forms (PMID: 9642297).