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验证数据展示

  • WB analysis using 68618-1-Ig

    WB analysis using 68618-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68618-1-Ig (ANGPT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of HUVEC using 68618-1-Ig

    IF Staining of HUVEC using 68618-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using Angiopoietin 1 antibody (68618-1-Ig, Clone: 3C4A3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

Angiopoietin 1 Monoclonal antibody

Angiopoietin 1 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68618-1-Ig

产品说明书

CloneNo. 3C4A3

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

别名

ANGPT1, ANG-1, ANG1, ANG 1, AGPT

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inA375 cells, PNGase F treated A375 cells, non-treated U-87 MG cells, PNGase F treated U-87 MG cells, non-treated HuH-7 cells, PNGase F treated HuH-7 MG cells, non-treated bENd.3 cells, PNGase F treated bENd.3 cells
Positive IF/ICC detected inHUVEC cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68618-1-Ig targets Angiopoietin 1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 Angiopoietin 1 fusion protein Ag18829 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 angiopoietin 1
别名 ANGPT1, ANG-1, ANG1, ANG 1, AGPT
计算分子量 498 aa, 58 kDa
观测分子量70 kDa, 55 kDa
GenBank蛋白编号BC152411
基因名称 Angiopoietin 1
Gene ID (NCBI) 284
RRIDAB_3085311
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ15389
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Angiopoietin 1 is a 70-kDa secreted glycoprotein generated from vascular mural cells, pericytes, and certain other cells. Angiopoietin 1 participates in vascular differentiation through angiogenesis, which is the process of the growth and remodelling of existing vessels. Angiopoietin 1 is also involved in the maintenance and turnover of blood vessels in mature animals. Angiopoietin 1 binds to and activates the TEK/TIE2 receptor by inducing its dimerization and tyrosine phosphorylation, playing an important role in the regulation of angiogenesis. Overexpression of Angiopoietin 1 has been proven to occur in malignant glioblastoma, neuroblastoma, non-small cell lung cancer, and other tumors.

实验方案

Product Specific Protocols
WB protocol for Angiopoietin 1 antibody 68618-1-IgDownload protocol
IF protocol for Angiopoietin 1 antibody 68618-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols