• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Angiopoietin-like 3/ANGPTL3 Recombinant antibody

Angiopoietin-like 3/ANGPTL3 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

230498F11

Cat no : 83165-3-RR

Print datasheet

Synonyms

angiopoietin like 3, Angptl3, hypl

验证数据展示

  • WB analysis using 83165-3-RR

    WB analysis using 83165-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83165-3-RR (Angiopoietin-like 3/ANGPTL3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of A375 using 83165-3-RR

    WB analysis of A375 using 83165-3-RR

    A375 cells were subjected to SDS PAGE followed by western blot with 83165-3-RR (Angiopoietin-like 3/ANGPTL3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHepG2 cells, A375 cells, HEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83165-3-RR targets Angiopoietin-like 3/ANGPTL3 in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name angiopoietin-like 3
Synonyms angiopoietin like 3, Angptl3, hypl
Calculated Molecular Weight53KD
Observed Molecular Weight 54 kDa, 70 kDa
GenBank Accession NumberNM_013913.4
Gene Symbol Angptl3
Gene ID (NCBI) 30924
RRIDAB_3670860
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9R182
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ANGPTL3 belongs to the angiopoietin-like protein family. ANGPTL3 is mainly synthesized by liver cells and is notably expressed in kidney podocytes. Accumulating evidences have revealed that ANGPTL3 plays a critical role in both biological processes, such as lipid metabolism, angiogenesis and haematopoietic function and pathological changes, including atherosclerosis, carcinogenesis, nephrotic syndrome, diabetes, liver diseases and so on. Thus, ANGPTL3 may serve as a potential biomarker in these diseases. ANGPTL3 belongs to a multifunctional secreted protein that mainly expresses in the liver, and is regulated by numerous post-translational modifications, including multiple cleavage and glycosylation (PMID: 30615486).

实验方案

Product Specific Protocols
WB protocol for Angiopoietin-like 3/ANGPTL3 antibody 83165-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols