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验证数据展示

  • WB analysis of mouse small intestine using 25153-1-AP

    WB analysis of mouse small intestine using 25153-1-AP

    mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 25153-1-AP (Annexin A13 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse skeletal muscle using 25153-1-AP

    WB analysis of mouse skeletal muscle using 25153-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 25153-1-AP (Annexin A13 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human small intestine using 25153-1-AP

    IHC staining of human small intestine using 25153-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 25153-1-AP (Annexin A13 antibody at dilution of 1:50 (under 10x lens).

  • IHC staining of human small intestine using 25153-1-AP

    IHC staining of human small intestine using 25153-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 25153-1-AP (Annexin A13 antibody at dilution of 1:50 (under 40x lens).

Annexin A13 Polyclonal antibody

Annexin A13 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 25153-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

Annexin 13, annexin A13, Annexin XIII, ANX13, ANXA13, Intestine specific annexin, ISA

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse skeletal muscle tissue, mouse small intestine tissue
Positive IHC detected inhuman small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25153-1-AP targets Annexin A13 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 Annexin A13 fusion protein Ag18581 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 annexin A13
别名 Annexin 13, annexin A13, Annexin XIII, ANX13, ANXA13, Intestine specific annexin, ISA
计算分子量 316 aa, 35 kDa
观测分子量 35 kDa / 40 kDa
GenBank蛋白编号BC125158
基因名称 Annexin A13
Gene ID (NCBI) 312
RRIDAB_2879928
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP27216
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Annexin A13 antibody 25153-1-APDownload protocol
IHC protocol for Annexin A13 antibody 25153-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols