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  • Featured Product
  • KD/KO Validated

Annexin A2 Monoclonal antibody, PBS Only

Annexin A2 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1C1E12

Cat no : 66035-1-PBS

Print datasheet

Synonyms

ANXA2, ANX2L4, ANX2, Annexin-2, Annexin II

验证数据展示

  • WB analysis using 66035-1-Ig (same clone as 66035-1-PBS)

    WB analysis using 66035-1-Ig (same clone as 66035-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66035-1-Ig (Annexin A2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66035-1-PBS in a different storage buffer formulation.

  • WB analysis using 66035-1-Ig (same clone as 66035-1-PBS)

    WB analysis using 66035-1-Ig (same clone as 66035-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66035-1-Ig (Annexin A2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66035-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 66035-1-Ig (same clone as 66035-1-PBS)

    WB analysis of HEK-293 using 66035-1-Ig (same clone as 66035-1-PBS)

    WB result of Annexin A2 antibody (66035-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Annexin A2 transfected HEK-293 cells. This data was developed using the same antibody clone with 66035-1-PBS in a different storage buffer formulation.

  • IF Staining of T-47D using 66035-1-Ig (same clone as 66035-1-PBS)

    IF Staining of T-47D using 66035-1-Ig (same clone as 66035-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed T-47D cells using 66035-1-Ig (Annexin A2 antibody), at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66035-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66035-1-Ig (same clone as 66035-1-PBS)

    IHC staining of human breast cancer using 66035-1-Ig (same clone as 66035-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66035-1-Ig (Annexin A2 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66035-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ANXA2 antibody included
  • IHC staining of human breast cancer using 66035-1-Ig (same clone as 66035-1-PBS)

    IHC staining of human breast cancer using 66035-1-Ig (same clone as 66035-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66035-1-Ig (Annexin A2 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66035-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ANXA2 antibody included
  • FC experiment of HeLa using 66035-1-Ig (same clone as 66035-1-PBS)

    FC experiment of HeLa using 66035-1-Ig (same clone as 66035-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Annexin A2 Monoclonal antibody (66035-1-Ig, Clone:1C1E12) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66035-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy ANXA2 Ready-To-Use IHC Kit. ANXA2 primary antibody included.

产品信息

66035-1-PBS targets Annexin A2 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Annexin A2 fusion protein Ag16654 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name annexin A2
Synonyms ANXA2, ANX2L4, ANX2, Annexin-2, Annexin II
Calculated Molecular Weight 339 aa, 39 kDa
Observed Molecular Weight 39 kDa
GenBank Accession NumberBC016774
Gene Symbol Annexin A2
Gene ID (NCBI) 302
ENSEMBL Gene IDENSG00000182718
RRIDAB_11045659
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP07355
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Annexin A2 (ANXA2), also named Annexin-2 or Lipocortin II, is a Ca2+ binding protein that is up-regulated in virally transformed cell lines and human tumors. This calcium-regulated membrane-binding protein, whose affinity for calcium is greatly enhanced by anionic phospholipids, may cross-link plasma membrane phospholipids with actin and the cytoskeleton and be involved with exocytosis. Heat-stress response may also involve Annexin-2.