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  • Featured Product
  • KD/KO Validated

Annexin A2 Monoclonal antibody, PBS Only

Annexin A2 Monoclonal Antibody for WB,IHC,IF,FC,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB,IHC,IF,FC,ELISA

Conjugate

Unconjugated

CloneNo.

1C1E12

Cat No : 66035-1-PBS

Print datasheet

Synonyms

Annexin 2, annexin A2, Annexin II, ANX2, ANX2L4, ANXA2, CAL1H, Calpactin 1 heavy chain, Calpactin I heavy chain, Chromobindin 8, LIP2, Lipocortin II, LPC2, LPC2D, P36, PAP IV, Protein I

验证数据展示

  • WB analysis using 66035-1-Ig (same clone as 66035-1-PBS)

    WB analysis using 66035-1-Ig (same clone as 66035-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66035-1-Ig (Annexin A2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66035-1-PBS in a different storage buffer formulation.

  • WB analysis using 66035-1-Ig (same clone as 66035-1-PBS)

    WB analysis using 66035-1-Ig (same clone as 66035-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66035-1-Ig (Annexin A2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66035-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 66035-1-Ig (same clone as 66035-1-PBS)

    WB analysis of HEK-293 using 66035-1-Ig (same clone as 66035-1-PBS)

    WB result of Annexin A2 antibody (66035-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Annexin A2 transfected HEK-293 cells. This data was developed using the same antibody clone with 66035-1-PBS in a different storage buffer formulation.

  • IF Staining of T-47D using 66035-1-Ig (same clone as 66035-1-PBS)

    IF Staining of T-47D using 66035-1-Ig (same clone as 66035-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed T-47D cells using 66035-1-Ig (Annexin A2 antibody), at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66035-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66035-1-Ig (same clone as 66035-1-PBS)

    IHC staining of human breast cancer using 66035-1-Ig (same clone as 66035-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66035-1-Ig (Annexin A2 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66035-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ANXA2 antibody included
  • IHC staining of human breast cancer using 66035-1-Ig (same clone as 66035-1-PBS)

    IHC staining of human breast cancer using 66035-1-Ig (same clone as 66035-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66035-1-Ig (Annexin A2 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66035-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ANXA2 antibody included
  • FC experiment of K-562 using 66035-1-Ig (same clone as 66035-1-PBS)

    FC experiment of K-562 using 66035-1-Ig (same clone as 66035-1-PBS)

    1X10^6 K-562 cells were stained with 0.2ug Annexin A2 antibody (66035-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1500. This data was developed using the same antibody clone with 66035-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy ANXA2 Ready-To-Use IHC Kit. ANXA2 primary antibody included.

产品信息

66035-1-PBS targets Annexin A2 in WB, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB,IHC,IF,FC,ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Annexin A2 fusion protein Ag16654 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name annexin A2
Synonyms Annexin 2, annexin A2, Annexin II, ANX2, ANX2L4, ANXA2, CAL1H, Calpactin 1 heavy chain, Calpactin I heavy chain, Chromobindin 8, LIP2, Lipocortin II, LPC2, LPC2D, P36, PAP IV, Protein I
Calculated Molecular Weight 339 aa, 39 kDa
Observed Molecular Weight 39 kDa
GenBank Accession NumberBC016774
Gene Symbol ANXA2
Gene ID (NCBI) 302
ENSEMBL Gene IDENSG00000182718
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP07355
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

背景介绍

Annexin A2 (ANXA2), also named Annexin-2 or Lipocortin II, is a Ca2+ binding protein that is up-regulated in virally transformed cell lines and human tumors. This calcium-regulated membrane-binding protein, whose affinity for calcium is greatly enhanced by anionic phospholipids, may cross-link plasma membrane phospholipids with actin and the cytoskeleton and be involved with exocytosis. Heat-stress response may also involve Annexin-2.