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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated Annexin A2 Monoclonal antibody

Annexin A2 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1C1E12

Cat no : CL488-66035

Print datasheet

Synonyms

Annexin 2, annexin A2, Annexin II, ANX2, ANX2L4, ANXA2, CAL1H, Calpactin 1 heavy chain, Calpactin I heavy chain, Chromobindin 8, LIP2, Lipocortin II, LPC2, LPC2D, P36, PAP IV, Protein I

验证数据展示

  • IF Staining of T-47D using CL488-66035

    IF Staining of T-47D using CL488-66035

    Immunofluorescent analysis of (-20°C Ethanol) fixed T-47D cells using CoraLite® Plus 488-conjugated Annexin A2 antibody (CL488-66035, Clone: 1C1E12 ) at dilution of 1:600.

经过测试的应用

Positive IF/ICC detected inT-47D cells
Planning an IHC experiment? We recommend our IHCeasy ANXA2 Ready-To-Use IHC Kit. ANXA2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66035-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of CL488-66035 is Annexin A2 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC Application Description
Tested Reactivity human
Immunogen Annexin A2 fusion protein Ag16654 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name annexin A2
Synonyms Annexin 2, annexin A2, Annexin II, ANX2, ANX2L4, ANXA2, CAL1H, Calpactin 1 heavy chain, Calpactin I heavy chain, Chromobindin 8, LIP2, Lipocortin II, LPC2, LPC2D, P36, PAP IV, Protein I
Calculated Molecular Weight 339 aa, 39 kDa
Observed Molecular Weight 39 kDa
GenBank Accession NumberBC016774
Gene Symbol Annexin A2
Gene ID (NCBI) 302
ENSEMBL Gene IDENSG00000182718
RRIDAB_2919279
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP07355
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Annexin A2 (ANXA2), also named Annexin-2 or Lipocortin II, is a Ca2+ binding protein that is up-regulated in virally transformed cell lines and human tumors. This calcium-regulated membrane-binding protein, whose affinity for calcium is greatly enhanced by anionic phospholipids, may cross-link plasma membrane phospholipids with actin and the cytoskeleton and be involved with exocytosis. Heat-stress response may also involve Annexin-2.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Annexin A2 antibody CL488-66035Download protocol
Standard Protocols
Click here to view our Standard Protocols