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验证数据展示

  • WB analysis using 29508-1-AP

    WB analysis using 29508-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29508-1-AP (Annexin A4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis using 29508-1-AP

    WB analysis using 29508-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29508-1-AP (Annexin IV antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Annexin A4 Polyclonal antibody

Annexin A4 Polyclonal Antibody for WB, ELISA
Cat No. 29508-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, rat, mouse

应用

WB, ELISA

别名

35 beta calcimedin, Annexin 4, annexin A4, Annexin IV, ANX4, ANXA4, Chromobindin 4, DKFZp686H02120, Endonexin I, Lipocortin IV, P32.5, PAP II, PIG28, PP4 X, Protein II, ZAP36

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, mouse kidney tissue, mouse liver tissue, mouse lung tissue, rat kidney tissue, NIH/3T3 cells, RAW 264.7 cells, HuH-7 cells, PC-12 cells, C6 cells, human placenta tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29508-1-AP targets Annexin A4 in WB, ELISA applications and shows reactivity with Human, rat, mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human, rat, mouse
免疫原 Annexin A4 fusion protein Ag30827 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 annexin A4
别名 35 beta calcimedin, Annexin 4, annexin A4, Annexin IV, ANX4, ANXA4, Chromobindin 4, DKFZp686H02120, Endonexin I, Lipocortin IV, P32.5, PAP II, PIG28, PP4 X, Protein II, ZAP36
计算分子量 36 kDa
观测分子量36 kDa
GenBank蛋白编号BC000182
基因名称 Annexin A4
Gene ID (NCBI) 307
RRIDAB_2918319
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP09525
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

Annexin A4 (Anxa4) is one of the Ca2 +-regulated and phospholipid-binding annexin superfamily proteins, and is located at 2p13. Anxa4 has a potential role in diagnosis, prognosis, and treatment of certain cancers. Annexin A4 shares 45 to 59% identity with other members and possibly interacts with ATP. Annexin A4 has in vitro anticoagulant activity and also inhibits phospholipase A2 activity. The Annexin A4 protein is expressed in a wide range of tissues, associated with polarized epithelial cells and is proposed to be involved in the regulation of chloride ions across the epithelial membrane.

实验方案

Product Specific Protocols
WB protocol for Annexin A4 antibody 29508-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols