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验证数据展示

  • WB analysis using 67461-1-Ig (same clone as 67461-1-PBS)

    WB analysis using 67461-1-Ig (same clone as 67461-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67461-1-Ig (ARFIP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67461-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 67461-1-Ig (same clone as 67461-1-PBS)

    IHC staining of mouse kidney using 67461-1-Ig (same clone as 67461-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 67461-1-Ig (Arfaptin-1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67461-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ARFIP1 antibody included
  • IHC staining of mouse kidney using 67461-1-Ig (same clone as 67461-1-PBS)

    IHC staining of mouse kidney using 67461-1-Ig (same clone as 67461-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 67461-1-Ig (Arfaptin-1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67461-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ARFIP1 antibody included
  • IF Staining of mouse kidney using 67461-1-Ig (same clone as 67461-1-PBS)

    IF Staining of mouse kidney using 67461-1-Ig (same clone as 67461-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using Arfaptin-1 antibody (67461-1-Ig, Clone: 2A10B3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67461-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse kidney using 67461-1-Ig (same clone as 67461-1-PBS)

    IF Staining of mouse kidney using 67461-1-Ig (same clone as 67461-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using Arfaptin-1 antibody (67461-1-Ig, Clone: 2A10B3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67461-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 67461-1-Ig (same clone as 67461-1-PBS)

    IF Staining of HeLa using 67461-1-Ig (same clone as 67461-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Arfaptin-1 antibody (67461-1-Ig, Clone: 2A10B3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67461-1-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 67461-1-Ig (same clone as 67461-1-PBS)

    IF Staining of A549 using 67461-1-Ig (same clone as 67461-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using Arfaptin-1 antibody (67461-1-Ig, Clone: 2A10B3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67461-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP51228-2

    Cytometric bead array standard curve of MP51228-2

    Cytometric bead array standard curve of MP51228-2, Arfaptin-1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67461-3-PBS. Detection antibody: 67461-1-PBS. Standard:Ag12097. Range: 3.125-100 ng/mL.

Arfaptin-1 Monoclonal antibody, PBS Only (Detector)

Arfaptin-1 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA
Cat No. 67461-1-PBS
CloneNo. 2A10B3

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA

Synonyms

ARFIP1, Arfaptin 1, ADP-ribosylation factor-interacting protein 1, 2A10B3

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 
100 μg

¥5800/ 100 μg

浓度: 1mg/ml

库存：1周货期

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Planning an IHC experiment? We recommend our IHCeasy ARFIP1 Ready-To-Use IHC Kit. ARFIP1 primary antibody included.

产品信息

67461-1-PBS targets Arfaptin-1 as part of a matched antibody pair:

MP51228-2: 67461-3-PBS capture and 67461-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Arfaptin-1 fusion protein Ag12097 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name ADP-ribosylation factor interacting protein 1
Synonyms ARFIP1, Arfaptin 1, ADP-ribosylation factor-interacting protein 1, 2A10B3
Calculated Molecular Weight 373 aa, 42 kDa
Observed Molecular Weight 38-42 kDa
GenBank Accession NumberBC103759
Gene Symbol Arfaptin-1
Gene ID (NCBI) 27236
RRIDAB_2882691
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP53367
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C