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验证数据展示

  • WB analysis using 82975-1-RR

    WB analysis using 82975-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82975-1-RR (Arginase-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse liver using 82975-1-RR

    IF Staining of mouse liver using 82975-1-RR

    Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using Arginase-1 antibody (82975-1-RR, Clone: 4M7 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IHC staining of mouse liver using 82975-1-RR

    IHC staining of mouse liver using 82975-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 82975-1-RR (Arginase-1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ARG1 antibody included
  • IF Staining of HepG2 using 82975-1-RR

    IF Staining of HepG2 using 82975-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Arginase-1 antibody (82975-1-RR, Clone: 4M7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 82975-1-RR

    Affinity and Kinetic Characterization of 82975-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82975-1-RR against Human Arginase-1 were performed. The affinity constant is 4.77 nM.

Arginase-1 Recombinant antibody

Arginase-1 Recombinant Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Cat No. 82975-1-RR

产品说明书

CloneNo. 4M7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IF-P, ELISA

别名

ARG1, Arginase 1, liver Arginase, arginase, liver, Arg-1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse liver tissue, rat liver tissue
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse liver tissue
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy ARG1 Ready-To-Use IHC Kit. ARG1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82975-1-RR targets Arginase-1 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Arginase-1 fusion protein Ag8595 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 arginase, liver
别名 ARG1, Arginase 1, liver Arginase, arginase, liver, Arg-1
计算分子量236aa,25 kDa; 322aa,35 kDa
观测分子量 35-36 kDa
GenBank蛋白编号BC005321
基因名称 Arginase-1
Gene ID (NCBI) 383
RRIDAB_3670723
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP05089
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Arginase-1 (Liver arginase) belongs to the arginase family. ARG1 is a novel immunohistochemical marker of hepatocellular differentiation in fine needle aspiration cytology and a marker of hepatocytes and hepatocellular neoplasms. ARG1 is closely associated with alternative macrophage activation and ARG1 has been shown to protectmotor neurons from trophic factor deprivation and allow sensory neurons to overcome neurite outgrowth inhibition by myelin proteins (PMID: 20071539, PMID:12098359). It can exsit as a homotrimer and it has 3 isoforms produced by alternative splicing (PMID:16141327). Defects in ARG1 are the cause of argininemia (ARGIN). Deletion or TNF-mediated restriction of ARG1 unleashes the production of NO by NOS2, which is critical for pathogen control (PMID:27117406). ARG1 mainly expresses in neurons in a normal brain. The expression of ARG1 increases in microglia/macrophages and astrocytes early after CNS injuries. ARG1 has been regarded as a marker for beneficial microglia/macrophages and possesses antiinflammatory and tissue repair properties under various pathological conditions (PMID: 26538310, PMID: 31619589).

实验方案

Product Specific Protocols
WB protocol for Arginase-1 antibody 82975-1-RRDownload protocol
IHC protocol for Arginase-1 antibody 82975-1-RRDownload protocol
IF protocol for Arginase-1 antibody 82975-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols