验证数据展示
经过测试的应用
Positive WB detected in | LNCaP cells, HeLa cells, HEK-293 cells, HepG2 cells, MCF-7 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells |
Positive IHC detected in | rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | rat brain tissue |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:2000-1:10000 |
Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
68316-1-Ig targets Ataxin 2 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
经测试应用 | WB, IHC, IF-P, ELISA Application Description |
经测试反应性 | Human, Mouse, Rat |
免疫原 | Ataxin 2 fusion protein Ag17402 种属同源性预测 |
宿主/亚型 | Mouse / IgM |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | ataxin 2 |
别名 | ataxin 2, ATX2, ATXN2, FLJ46772, SCA2, TNRC13 |
计算分子量 | 1313 aa, 140 kDa |
观测分子量 | 140-150 kDa |
GenBank蛋白编号 | BC114546 |
基因名称 | ATXN2 |
Gene ID (NCBI) | 6311 |
RRID | AB_3085052 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Caprylic acid/ammonium sulfate precipitation |
UNIPROT ID | Q99700 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ATXN2 contains a repeat structure with 22 or 23 triplets coding for glutamine and the (CAG)8CAA(CAG)4CAA(CAG)8 sequence; expansion of this domain to a size ≥34 triplets with a pure CAG sequence primarily causes autosomal dominant SCA2 [PMID:18418684], while ATXN2 expansions with CAA interruptions were observed as the cause of Levo-dopa responsive Parkinson's disease [PMID:10668726]. ATXN2 expansions associated with ALS were reported to be interrupted by at least one CAA triplet [PMID:21537950]
实验方案
Product Specific Protocols | |
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WB protocol for Ataxin 2 antibody 68316-1-Ig | Download protocol |
IHC protocol for Ataxin 2 antibody 68316-1-Ig | Download protocol |
IF protocol for Ataxin 2 antibody 68316-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |