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Atox1 Polyclonal antibody

Atox1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 31090-1-AP

Print datasheet

Synonyms

HAH1, Atx1

验证数据展示

  • WB analysis using 31090-1-AP

    WB analysis using 31090-1-AP

    NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 31090-1-AP (Atox1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse heart using 31090-1-AP

    IHC staining of mouse heart using 31090-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 31090-1-AP (Atox1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inNCI-H1299 cells
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31090-1-AP targets Atox1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Atox1 fusion protein Ag35290 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ATX1 (antioxidant protein 1) homolog 1 (yeast)
Synonyms HAH1, Atx1
Calculated Molecular Weight7 kDa
Observed Molecular Weight7-10 kDa
GenBank Accession NumberNM_009720
Gene Symbol Atox1
Gene ID (NCBI) 11927
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDO08997
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Antioxidant 1 copper chaperone (Atox1) is a small cytosolic protein containing an MBS (metal binding site) and a potential NLS (nuclear localisation signal). It plays an essential role in copper homeostasis by facilitating the transfer of copper to the trans-Golgi. Atox1 has been implicated in a wide range of diseases, including many neurodegenerative, cancer and metabolic disorders.

实验方案

Product Specific Protocols
WB protocol for Atox1 antibody 31090-1-APDownload protocol
IHC protocol for Atox1 antibody 31090-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols