AuthentiKine™ Human M-CSF ELISA Kit
Validation Data Gallery
Product Information
KE00184 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The M-CSF ELISA kit is to be used to detect and quantify protein levels of endogenous M-CSF. The assay recognizes human M-CSF. An antibody specific for M-CSF has been pre-coated onto the microwells. The M-CSF protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for M-CSF is added to detect the captured M-CSF protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | AuthentiKine™ Human M-CSF ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants, Urine, Saliva |
| Assay type | Sandwich |
| Sensitivity | 1.1 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 1435 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 82 | 71-88 |
| Cell culture supernatants | 85 | 80-94 |
| Urine | 95 | 89-101 |
| Saliva | 92 | 84-107 |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 207.3 | 5.5 | 2.7 |
| 2 | 20 | 433.8 | 8.8 | 2.0 |
| 3 | 20 | 797.3 | 51.7 | 6.5 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 214.6 | 6.9 | 3.2 |
| 2 | 24 | 475.3 | 22.1 | 4.7 |
| 3 | 24 | 880.9 | 48.0 | 5.0 |
Background Information
M-CSF, also called colony-stimulating factor-1 or CSF-1), is a haematopoietic growth factor, which controls survival, proliferation, differentiation and function of mononuclear phagocytes. It also plays a role in bone metabolism, fertility, pregnancy and inflammatory processes. M-CSF may be expressed on the surface of the cell as a membrane-spanning glycoprotein or secreted into circumstance. M-CSF was identified as an 84-kDa homodimeric glycoprotein found in healthy human urine, and is present in the peripheral blood and urine. M-CSF serum levels were elevated in pre-transplant haemodialysis patients, so it might be a specific marker of Kidney Allograft Rejection.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CSF 1, CSF1, Lanimostim, MCSF, M-CSF |
Publications
| Species | Sample Type | Title |
|---|---|---|
Cell Death Dis Blocking iASPP/Nrf2/M-CSF axis improves anti-cancer effect of chemotherapy-induced senescence by attenuating M2 polarization. | ||
Front Immunol Identification of biomarkers related to immune and inflammation in membranous nephropathy: comprehensive bioinformatic analysis and validation |