AuthentiKine™ Human M-CSF ELISA Kit0 Publications

Catalog number: KE00184

规格

单价

96T

¥3200

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Overview


Product name:
AuthentiKine™ Human M-CSF ELISA Kit

Tests:
1 X 96 well plate

Sample type:
Serum, Plasma, Cell culture supernatants, Urine, Saliva

Assay type:
Sandwich

Sensitivity:
1.1 pg/mL

Range:
15.6-1000 pg/mL

Reacted Species:
Human

Tested applications:
Sandwich ELISA

Recovery:
Sample TypeAverageRange
Human plasma 82 71-88
Cell culture supernatants 85 80-94
Urine 95 89-101
Saliva 92 84-107

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 207.3 5.5 2.7
2 20 433.8 8.8 2.0
3 20 797.3 51.7 6.5

InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 214.6 6.9 3.2
2 24 475.3 22.1 4.7
3 24 880.9 48.0 5.0

Product overview:
KE00184 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The M-CSF ELISA kit is to be used to detect and quantify protein levels of endogenous M-CSF. The assay recognizes human M-CSF. An antibody specific for M-CSF has been pre-coated onto the microwells. The M-CSF protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for M-CSF is added to detect the captured M-CSF protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.

Properties


Storage Instructions:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

Synonyms:
CSF 1, CSF1, Lanimostim, MCSF, M-CSF
Background

M-CSF, also called colony-stimulating factor-1 or CSF-1), is a haematopoietic growth factor, which controls survival, proliferation, differentiation and function of mononuclear phagocytes. It also plays a role in bone metabolism, fertility, pregnancy and inflammatory processes. M-CSF may be expressed on the surface of the cell as a membrane-spanning glycoprotein or secreted into circumstance. M-CSF was identified as an 84-kDa homodimeric glycoprotein found in healthy human urine, and is present in the peripheral blood and urine. M-CSF serum levels were elevated in pre-transplant haemodialysis patients, so it might be a specific marker of Kidney Allograft Rejection.


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