AuthentiKine™ Human CXCL8/IL-8 ELISA Kit
Validation Data Gallery
Product Information
KE00275 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human CXCL8/IL-8 ELISA kit is to be used to detect and quantify protein levels of endogenous CXCL8/IL-8. The assay recognizes human CXCL8/IL-8. An antibody specific for CXCL8/IL-8 has been pre-coated onto the microwells. The CXCL8/IL-8 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for human CXCL8/IL-8 is added to detect the captured human CXCL8/IL-8 protein. For signal development, HRP-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | AuthentiKine™ Human CXCL8/IL-8 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 0.2 pg/mL |
| Range | 1.56-100 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3576 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 107% | 76%-128% |
| Cell culture supernatants | 106% | 86%-121% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 86.5 | 3.8 | 4.4 |
| 2 | 20 | 19.9 | 0.3 | 1.6 |
| 3 | 20 | 5.0 | 0.1 | 2.9 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 82.0 | 4.2 | 5.1 |
| 2 | 24 | 19.3 | 0.7 | 3.4 |
| 3 | 24 | 4.5 | 0.3 | 7.4 |
Background Information
Interleukin 8 (IL-8), also known as CXCL8, which is a member of the CXC chemokine family. This chemokine is secreted by a variety of cell types including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, and various tumor cell lines in response to inflammatory stimuli. IL-8 has two primary functions. It induces chemotaxis in target cells, primarily neutrophils but also other granulocytes, causing them to migrate toward the site of infection. IL-8 also induces phagocytosis once they have arrived. This gene is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. IL-8 is also known to be a potent promoter of angiogenesis. IL-8 has been associated with tumor angiogenesis, metastasis, and poor prognosis in breast cancer. IL-8 may present a novel therapeutic target for estrogen driven breast carcinogenesis and tumor progression.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | IL8, IL 8, interleukin 8, IL-8, CXCL8 |
Publications
| Species | Sample Type | Title |
|---|---|---|
Sci Rep Protective effects of a new generation of probiotic Bacteroides fragilis against colitis in vitro and in vitro | ||
Adv Sci (Weinh) Exosomes-transferred LINC00668 Contributes to Thrombosis by Promoting NETs Formation in Inflammatory Bowel Disease | ||
Heliyon Abnormal expression of CEBPB promotes the progression of renal cell carcinoma through regulating the generation of IL-6 |