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B2M Monoclonal antibody, PBS Only (Detector)

B2M Monoclonal Antibody for Cytometric bead array, Indirect ELISA, Sample test

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

Cytometric bead array, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

4D4D5

Cat no : 68395-3-PBS

Print datasheet

Synonyms

Beta-2-microglobulin, HDCMA22P, CDABP0092, Beta-2-microglobulin form pI 5.3, beta 2 microglobulin

验证数据展示

  • Sample test of MP50108-1

    Sample test of MP50108-1

    Cytometric bead array of A375 cells supernatant using MP50108-1, B2M Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68395-2-PBS. Detection antibody: 68395-3-PBS. Gradient density (indicated at X axis) of A375 cells were inoculated in 96 well plate followed by cultured for 2 days. The supernatant was analyzed using MP50108-1 (B2M Monoclonal Matched Antibody Pair). The signal (Median Fluorescenence Intensity) is highly correlated with the cell density （refer to bottom X - left Y axis). The left over cells were analyzed using cell proliferation assay reagent MTS. Signal was measured by absorbance at 492nm (refer to top X - right Y axis).

  • Sample test of MP50108-1

    Sample test of MP50108-1

    Cytometric bead array of HeLa cells supernatant using MP50108-1, B2M Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68395-2-PBS. Detection antibody: 68395-3-PBS. Gradient density (indicated at X axis) of HeLa cells were inoculated in 96 well plate followed by cultured for 2 days. The supernatant was analyzed using MP50108-1 (B2M Monoclonal Matched Antibody Pair). The signal (Median Fluorescenence Intensity) is highly correlated with the cell density （refer to bottom X - left Y axis). The left over cells were analyzed using cell proliferation assay reagent MTS. Signal was measured by absorbance at 492nm (refer to top X - right Y axis).

  • Sample test of MP50108-1

    Sample test of MP50108-1

    Cytometric bead array of U-251 cells supernatant using MP50108-1, B2M Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68395-2-PBS. Detection antibody: 68395-3-PBS. Gradient density (indicated at X axis) of U-251 cells were inoculated in 96 well plate followed by cultured for 2 days. The supernatant was analyzed using MP50108-1 (B2M Monoclonal Matched Antibody Pair). The signal (Median Fluorescenence Intensity) is highly correlated with the cell density （refer to bottom X - left Y axis). The left over cells were analyzed using cell proliferation assay reagent MTS. Signal was measured by absorbance at 492nm (refer to top X - right Y axis).

  • Sample test of MP50108-1

    Sample test of MP50108-1

    Cytometric bead array of various cell lines supernatant using MP50108-1, B2M Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68395-2-PBS. Detection antibody: 68395-3-PBS. Supernatant (50μL/well) from the following cell lines were assayed: LNCaP, THP-1, Karpas299, BT-549, HT-29, U266B1, Kasumi-1, Jurkat, HCT116, TT, MCF-7, K-562, Raji, NCCIT, BxPC-3, PC-3, Ramos, A549, SK-N-SH, U-251, HepG2, Caco-2, 293, 293T, hTERT-RPE1, A431, HT-1080, Saos-2, SH-SY5Y, UC-MSCS. Complete culture medium was served as blank control.

  • Sample test of MP50108-1

    Sample test of MP50108-1

    Cytometric bead array of various cell lines supernatant using MP50108-1, B2M Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68395-2-PBS. Detection antibody: 68395-3-PBS. Supernatant (50μL/well) from the following cell lines were assayed: hTERT-RPE1, U251, A431, PC-12, ROS1728, NIH/3T3, mMSCS. Complete culture containing DMEM and FBS was served as blank control. Note that this matched antibody pair recognizes human B2M (beta 2 macroglobulin) but does not recognize rat or mouse B2M.

  • Cytometric bead array standard curve of MP50108-1

    Cytometric bead array standard curve of MP50108-1

    Cytometric bead array standard curve of MP50108-1, B2M Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68395-2-PBS. Detection antibody: 68395-3-PBS. Standard:Eg31815. Range: 0.098-100 ng/mL.

Planning an IHC experiment? We recommend our IHCeasy Beta-2-Microglobulin Ready-To-Use IHC Kit. Beta-2-Microglobulin primary antibody included.

产品信息

68395-3-PBS targets B2M as part of a matched antibody pair:

MP50108-1: 68395-2-PBS capture and 68395-3-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications Cytometric bead array, Indirect ELISA, Sample test Application Description
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name beta-2-microglobulin
Synonyms Beta-2-microglobulin, HDCMA22P, CDABP0092, Beta-2-microglobulin form pI 5.3, beta 2 microglobulin
Calculated Molecular Weight 119 aa, 14 kDa
GenBank Accession NumberBC032589
Gene Symbol B2M
Gene ID (NCBI) 567
ENSEMBL Gene IDENSG00000166710
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP61769
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C