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验证数据展示

  • Sample test of MP50108-1

    Sample test of MP50108-1

    Cytometric bead array of A375 cells supernatant using MP50108-1, B2M Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68395-2-PBS. Detection antibody: 68395-3-PBS. Gradient density (indicated at X axis) of A375 cells were inoculated in 96 well plate followed by cultured for 2 days. The supernatant was analyzed using MP50108-1 (B2M Monoclonal Matched Antibody Pair). The signal (Median Fluorescenence Intensity) is highly correlated with the cell density （refer to bottom X - left Y axis). The left over cells were analyzed using cell proliferation assay reagent MTS. Signal was measured by absorbance at 492nm (refer to top X - right Y axis).

  • Sample test of MP50108-1

    Sample test of MP50108-1

    Cytometric bead array of HeLa cells supernatant using MP50108-1, B2M Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68395-2-PBS. Detection antibody: 68395-3-PBS. Gradient density (indicated at X axis) of HeLa cells were inoculated in 96 well plate followed by cultured for 2 days. The supernatant was analyzed using MP50108-1 (B2M Monoclonal Matched Antibody Pair). The signal (Median Fluorescenence Intensity) is highly correlated with the cell density （refer to bottom X - left Y axis). The left over cells were analyzed using cell proliferation assay reagent MTS. Signal was measured by absorbance at 492nm (refer to top X - right Y axis).

  • Sample test of MP50108-1

    Sample test of MP50108-1

    Cytometric bead array of U-251 cells supernatant using MP50108-1, B2M Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68395-2-PBS. Detection antibody: 68395-3-PBS. Gradient density (indicated at X axis) of U-251 cells were inoculated in 96 well plate followed by cultured for 2 days. The supernatant was analyzed using MP50108-1 (B2M Monoclonal Matched Antibody Pair). The signal (Median Fluorescenence Intensity) is highly correlated with the cell density （refer to bottom X - left Y axis). The left over cells were analyzed using cell proliferation assay reagent MTS. Signal was measured by absorbance at 492nm (refer to top X - right Y axis).

  • Sample test of MP50108-1

    Sample test of MP50108-1

    Cytometric bead array of various cell lines supernatant using MP50108-1, B2M Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68395-2-PBS. Detection antibody: 68395-3-PBS. Supernatant (50μL/well) from the following cell lines were assayed: LNCaP, THP-1, Karpas299, BT-549, HT-29, U266B1, Kasumi-1, Jurkat, HCT116, TT, MCF-7, K-562, Raji, NCCIT, BxPC-3, PC-3, Ramos, A549, SK-N-SH, U-251, HepG2, Caco-2, 293, 293T, hTERT-RPE1, A431, HT-1080, Saos-2, SH-SY5Y, UC-MSCS. Complete culture medium was served as blank control.

  • Sample test of MP50108-1

    Sample test of MP50108-1

    Cytometric bead array of various cell lines supernatant using MP50108-1, B2M Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68395-2-PBS. Detection antibody: 68395-3-PBS. Supernatant (50μL/well) from the following cell lines were assayed: hTERT-RPE1, U251, A431, PC-12, ROS1728, NIH/3T3, mMSCS. Complete culture containing DMEM and FBS was served as blank control. Note that this matched antibody pair recognizes human B2M (beta 2 macroglobulin) but does not recognize rat or mouse B2M.

  • Cytometric bead array standard curve of MP50108-1

    Cytometric bead array standard curve of MP50108-1

    Cytometric bead array standard curve of MP50108-1, B2M Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68395-2-PBS. Detection antibody: 68395-3-PBS. Standard:Eg31815. Range: 0.098-100 ng/mL.

Beta-2-Microglobulin Monoclonal antibody, PBS Only (Detector)

Beta-2-Microglobulin Monoclonal Antibody for Cytometric bead array, Indirect ELISA, Sample test
Cat No. 68395-3-PBS

产品说明书

CloneNo. 4D4D5

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

Cytometric bead array, Indirect ELISA, Sample test

别名

B2M, 4D4D5, CDABP0092, Beta-2-microglobulin form pI 5.3, beta 2-Microglobulin

缓冲液配方:  PBS Only
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy Beta-2-Microglobulin Ready-To-Use IHC Kit. Beta-2-Microglobulin primary antibody included.

产品信息

68395-3-PBS targets Beta-2-Microglobulin as part of a matched antibody pair:

MP50108-1: 68395-2-PBS capture and 68395-3-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 Cytometric bead array, Indirect ELISA, Sample test Application Description
经测试反应性 human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 beta-2-microglobulin
别名 B2M, 4D4D5, CDABP0092, Beta-2-microglobulin form pI 5.3, beta 2-Microglobulin
计算分子量 119 aa, 14 kDa
GenBank蛋白编号BC032589
基因名称 B2M
Gene ID (NCBI) 567
ENSEMBL Gene IDENSG00000166710
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP61769
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C