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B3GALNT1 Polyclonal antibody

B3GALNT1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22048-1-AP

Print datasheet

Synonyms

b3Gal T3, B3GALNT1, B3GALT3, Beta 1,3 GalNAc T1, Beta 1,3 GalTase 3, Beta 3 Gx T3, beta3Gal T3, Beta3GalT3, galT3, Gb4Cer, GLCT3, GLOB, Globoside synthase, P1

验证数据展示

  • WB analysis using 22048-1-AP

    WB analysis using 22048-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22048-1-AP (B3GALNT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse placenta using 22048-1-AP

    WB analysis of mouse placenta using 22048-1-AP

    mouse placenta tissue were subjected to SDS PAGE followed by western blot with 22048-1-AP (B3GALNT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human heart using 22048-1-AP

    IHC staining of human heart using 22048-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 22048-1-AP (B3GALNT1 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human heart using 22048-1-AP

    IHC staining of human heart using 22048-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 22048-1-AP (B3GALNT1 antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inmouse placenta tissue, MCF-7 cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman heart tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:300-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22048-1-AP targets B3GALNT1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen B3GALNT1 fusion protein Ag16976 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name beta-1,3-N-acetylgalactosaminyltransferase 1 (globoside blood group)
Synonyms b3Gal T3, B3GALNT1, B3GALT3, Beta 1,3 GalNAc T1, Beta 1,3 GalTase 3, Beta 3 Gx T3, beta3Gal T3, Beta3GalT3, galT3, Gb4Cer, GLCT3, GLOB, Globoside synthase, P1
Calculated Molecular Weight 40 kDa
Observed Molecular Weight 40-45 kDa
GenBank Accession NumberBC047618
Gene Symbol B3GALNT1
Gene ID (NCBI) 8706
RRIDAB_11182184
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75752
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for B3GALNT1 antibody 22048-1-APDownload protocol
IHC protocol for B3GALNT1 antibody 22048-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols