验证数据展示
经过测试的应用
| Positive WB detected in | PC-12 cells, SH-SY5Y cells |
| Positive IHC detected in | mouse brain tissue, mouse spleen tissue, human thyroid cancer tissue, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 4 publications below |
| IF | See 1 publications below |
产品信息
19401-1-AP targets B3GAT1 in WB, IHC, ELISA, IF applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | IHC, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | B3GAT1 fusion protein Ag13550 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P) |
| 别名 | GlcUAT-P, GlcAT-P, GLCATP, Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1, EC:2.4.1.135 |
| 计算分子量 | 38 kDa |
| 观测分子量 | 45 kDa, 70 kDa |
| GenBank蛋白编号 | BC010466 |
| 基因名称 | B3GAT1 |
| Gene ID (NCBI) | 27087 |
| RRID | AB_2878577 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9P2W7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
B3GAT1 is associated with glycosaminoglycan biosynthesis. It plays a key role in neurodevelopment. The calculated molecular weight of B3GAT1 is 38 kDa and 19401-1-AP antibody detectes 45 and 70 kDa forms in SDS-PAGE which might as a result of different degrees of glycosylation or the formation of homodimer.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for B3GAT1 antibody 19401-1-AP | Download protocol |
| IHC protocol for B3GAT1 antibody 19401-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancers (Basel) The Immunological Contribution of a Novel Metabolism-Related Signature to the Prognosis and Anti-Tumor Immunity in Cervical Cancer. | ||
Gynecol Oncol Increased co-expression of MEST and BRCA1 is associated with worse prognosis and immune infiltration in ovarian cancer. | ||
Front Bioeng Biotechnol Small-Molecule Induction Promotes Corneal Endothelial Cell Differentiation From Human iPS Cells. | ||
BMC Med Genomics Establishment and validation of an immune infiltration predictive model for ovarian cancer | ||
Aging (Albany NY) Single-cell and bulk sequencing analyses reveal the immune suppressive role of PTPN6 in glioblastoma |
