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Host
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Conjugate
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验证数据展示

  • WB analysis using 66711-1-Ig (same clone as 66711-1-PBS)

    WB analysis using 66711-1-Ig (same clone as 66711-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66711-1-Ig (B3GAT1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66711-1-PBS in a different storage buffer formulation.

  • WB analysis of mouse brain using 66711-1-Ig (same clone as 66711-1-PBS)

    WB analysis of mouse brain using 66711-1-Ig (same clone as 66711-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 66711-1-Ig (B3GAT1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66711-1-PBS in a different storage buffer formulation.

  • FC experiment of K-562 using 66711-1-Ig (same clone as 66711-1-PBS)

    FC experiment of K-562 using 66711-1-Ig (same clone as 66711-1-PBS)

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human CD57 (66711-1-Ig, Clone:1F4A11) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66711-1-PBS in a different storage buffer formulation.

B3GAT1 Monoclonal antibody, PBS Only

B3GAT1 Monoclonal Antibody for WB, FC (Intra), ELISA
Cat No. 66711-1-PBS

产品说明书

CloneNo. 1F4A11

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse

应用

WB, FC (Intra), ELISA

别名

GlcAT-P, GLCATP, Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1, EC:2.4.1.135, Beta-1,3-glucuronyltransferase 1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66711-1-PBS targets B3GAT1 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原 B3GAT1 fusion protein Ag13593 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P)
别名 GlcAT-P, GLCATP, Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1, EC:2.4.1.135, Beta-1,3-glucuronyltransferase 1
计算分子量 38 kDa
观测分子量 38 kDa, 100 kDa
GenBank蛋白编号BC010466
基因名称 B3GAT1
Gene ID (NCBI) 27087
RRIDAB_2882062
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9P2W7
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

B3GAT1 is associated with glycosaminoglycan biosynthesis. It plays a key role in neurodevelopment. The calculated molecular weight of B3GAT1 is 38 kDa and 66711-1-Ig antibody detectes 100 kDa form in SDS-PAGE which might as a result of homodimer or glycosylation.