• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

B3GNT2 Polyclonal antibody

B3GNT2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 14200-1-AP

Print datasheet

Synonyms

b3Gal T7, B3GALT7, B3GN T1, B3GN T2, B3GNT, B3GNT 2, B3GNT1, B3GNT2, Beta 1,3 GalTase 7, Beta 1,3 Gn T1, Beta 1,3 Gn T2, Beta 3 Gx T7, Beta3Gal T7, Beta3GalT7, Beta3Gn T1, Beta3Gn T2, BETA3GNT, BGnT 1, BGnT 2

验证数据展示

  • WB analysis using 14200-1-AP

    WB analysis using 14200-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14200-1-AP (B3GNT2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inJurkat cells, MCF-7 cells, SMMC-7721 cells, mouse brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14200-1-AP targets B3GNT2 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse
Immunogen B3GNT2 fusion protein Ag5404 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2
Synonyms b3Gal T7, B3GALT7, B3GN T1, B3GN T2, B3GNT, B3GNT 2, B3GNT1, B3GNT2, Beta 1,3 GalTase 7, Beta 1,3 Gn T1, Beta 1,3 Gn T2, Beta 3 Gx T7, Beta3Gal T7, Beta3GalT7, Beta3Gn T1, Beta3Gn T2, BETA3GNT, BGnT 1, BGnT 2
Calculated Molecular Weight 9 kDa
Observed Molecular Weight69 kDa
GenBank Accession NumberBC030579
Gene Symbol B3GNT2
Gene ID (NCBI) 10678
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NY97
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

β-1,3-N-acetylglucosaminyltransferase (B3GNT2) is the most abundant and ubiquitously expressed B3GNT isoform and displays the greatest in vitro activity for N-glycan and O-glycan poly-LacNAc extensionis. B3GNT2 encodes a poly-N-acetyllactosamine synthase that targets >10 ligands and receptors to disrupt interactions between tumor and T cells and reduce T cell activation (PMID: 35338135, 32663509). B3GNT2 also has five predicted N-glycosylation sites at asparagine residues 79, 89, 127, 173, and 219 (PMID: 33229435). Western blots indicated the glycosylated form of B3GNT2 is detected at ~ 67 kDa.

实验方案

Product Specific Protocols
WB protocol for B3GNT2 antibody 14200-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols