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B3GNT7 Polyclonal antibody

B3GNT7 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22879-1-AP

Print datasheet

Synonyms

BGnT7, Beta3Gn-T7, beta3GnT7, Beta-1,3-N-acetylglucosaminyltransferase 7, Beta-1,3-Gn-T7

验证数据展示

  • WB analysis of Jurkat using 22879-1-AP

    WB analysis of Jurkat using 22879-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 22879-1-AP (B3GNT7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 22879-1-AP

    IHC staining of human colon cancer using 22879-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22879-1-AP (B3GNT7 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inJurkat cells, COLO 320 cells, mouse placenta tissue
Positive IHC detected inhuman colon cancer tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:100-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22879-1-AP targets B3GNT7 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen B3GNT7 fusion protein Ag18890 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 7
Synonyms BGnT7, Beta3Gn-T7, beta3GnT7, Beta-1,3-N-acetylglucosaminyltransferase 7, Beta-1,3-Gn-T7
Calculated Molecular Weight 330 aa, 37 kDa
Observed Molecular Weight 40-46 kDa
GenBank Accession NumberBC148680
Gene Symbol B3GNT7
Gene ID (NCBI) 93010
RRIDAB_2879182
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NFL0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

B3GNT7, the glycosyltransferase gene that was most significantly upregulated by IL-22 treatment, encodes an N-acetylglucosaminyltransferase involved in the biosynthesis of polyLacNAc repeats of keratan sulfate (PMID: 34864058). B3GNT7 was initially identified in 2002 and is notable for high expression in healthy intestinal epithelial cells (PMID: 38886669). Overexpressed B3GNT7 were correlated with poor prognosis in breast cancer patients based on public datasets. B3GNT7 was shown to be a potential biomarker for unfavorable outcomes and therapeutic target of breast cancer (PMID: 37740763).

实验方案

Product Specific Protocols
WB protocol for B3GNT7 antibody 22879-1-APDownload protocol
IHC protocol for B3GNT7 antibody 22879-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols