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验证数据展示

  • WB analysis of Jurkat using 24024-1-AP

    WB analysis of Jurkat using 24024-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 24024-1-AP (B4GALNT2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 24024-1-AP

    IHC staining of human colon using 24024-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 24024-1-AP (B4GALNT2 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

B4GALNT2 Polyclonal antibody

B4GALNT2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 24024-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

UDP-GalNAc:Neu5Aca2-3Galb-R b1,4-N-acetylgalactosaminyltransferase, Sd(a) beta-1,4-GalNAc transferase, GALGT2, EC:2.4.1.-, Beta-1,4 N-acetylgalactosaminyltransferase 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, COLO 320 cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:600-1:2400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24024-1-AP targets B4GALNT2 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 B4GALNT2 fusion protein Ag21166 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 beta-1,4-N-acetyl-galactosaminyl transferase 2
别名 UDP-GalNAc:Neu5Aca2-3Galb-R b1,4-N-acetylgalactosaminyltransferase, Sd(a) beta-1,4-GalNAc transferase, GALGT2, EC:2.4.1.-, Beta-1,4 N-acetylgalactosaminyltransferase 2
计算分子量 566 aa, 63 kDa
观测分子量 65 kDa
GenBank蛋白编号BC113677
基因名称 B4GALNT2
Gene ID (NCBI) 124872
RRIDAB_2879405
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen Affinity purified
UNIPROT IDQ8NHY0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

B4GALNT2, also named as GALGT2, catalyzes the last step in the biosynthesis of the human Sd(a) antigen, which is found on more than 90% of Caucasian red blood cells. It also catalyzes the last step in the biosynthesis of the Cad antigen, which is related both serologically and biochemically to the Sd(a) antigen. B4GALNT2 transfers a beta-1,4-linked GalNAc to the galactose residue of an alpha-2,3-sialylated chain. This antibody detects the 55-65 kDa B4GALNT2 protein.

实验方案

Product Specific Protocols
WB protocol for B4GALNT2 antibody 24024-1-APDownload protocol
IHC protocol for B4GALNT2 antibody 24024-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols