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B4GALT2 Polyclonal antibody

B4GALT2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20330-1-AP

Print datasheet

Synonyms

B4Gal T2, B4Gal T3, B4GALT2, Beta 1,4 GalTase 2, beta4Gal T2

验证数据展示

  • WB analysis of PC-3 using 20330-1-AP

    WB analysis of PC-3 using 20330-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 20330-1-AP (B4GALT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 20330-1-AP

    IHC staining of human colon cancer using 20330-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 20330-1-AP (B4GALT2 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human colon cancer using 20330-1-AP

    IHC staining of human colon cancer using 20330-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 20330-1-AP (B4GALT2 antibody) at dilution of 1:100 (under 40x lens).

经过测试的应用

Positive WB detected inPC-3 cells, mouse brain tissue, mouse large intestine tissue, mouse ovary tissue, mouse skeletal muscle tissue
Positive IHC detected inhuman colon cancer tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

20330-1-AP targets B4GALT2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen B4GALT2 fusion protein Ag14046 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 2
Synonyms B4Gal T2, B4Gal T3, B4GALT2, Beta 1,4 GalTase 2, beta4Gal T2
Calculated Molecular Weight 372 aa, 42 kDa
Observed Molecular Weight 42-45 kDa
GenBank Accession NumberBC002431
Gene Symbol B4GALT2
Gene ID (NCBI) 8704
RRIDAB_10693615
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO60909
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for B4GALT2 antibody 20330-1-APDownload protocol
IHC protocol for B4GALT2 antibody 20330-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Front Genet

Identification of a Novel Glycosyltransferase Prognostic Signature in Hepatocellular Carcinoma Based on LASSO Algorithm.

Authors - Zhiyang Zhou
View Article