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验证数据展示

  • WB analysis of HEK-293 using 66481-1-Ig (same clone as 66481-1-PBS)

    WB analysis of HEK-293 using 66481-1-Ig (same clone as 66481-1-PBS)

    HEK-293, PC-3, LNCaP, HeLa cells, and human spleen, human placenta, pig spleen tissues were subjected to SDS PAGE followed by western blot with 66481-1-Ig (B7-H3/CD276 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66481-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 66481-1-Ig (same clone as 66481-1-PBS)

    WB analysis of HEK-293 using 66481-1-Ig (same clone as 66481-1-PBS)

    WB result of B7-H3/CD276 antibody (66481-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with normal HEK-293 (sh-control ) and three independent sh-B7-H3 transfected HEK-293 cells. This data was developed using the same antibody clone with 66481-1-PBS in a different storage buffer formulation.

  • WB analysis of A431 using 66481-1-Ig (same clone as 66481-1-PBS)

    WB analysis of A431 using 66481-1-Ig (same clone as 66481-1-PBS)

    Untreated and PNGase F-treated lysates of A431 cells and PC-3 cells were subjected to SDS PAGE followed by western blot with 66481-1-Ig (B7-H3/CD276 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808). This data was developed using the same antibody clone with 66481-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 66481-1-Ig (same clone as 66481-1-PBS)

    IHC staining of human tonsillitis using 66481-1-Ig (same clone as 66481-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66481-1-Ig (B7-H3/CD276 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66481-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 66481-1-Ig (same clone as 66481-1-PBS)

    IHC staining of human tonsillitis using 66481-1-Ig (same clone as 66481-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66481-1-Ig (B7-H3/CD276 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66481-1-PBS in a different storage buffer formulation.

  • IF Staining of human placenta using 66481-1-Ig (same clone as 66481-1-PBS)

    IF Staining of human placenta using 66481-1-Ig (same clone as 66481-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using B7-H3/CD276 antibody (66481-1-Ig, Clone: 1E7D1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66481-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

B7-H3 Monoclonal antibody, PBS Only

B7-H3 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 66481-1-PBS

产品说明书

CloneNo. 1E7D1

宿主/亚型

Mouse / IgG2a

种属反应性

human, pig

应用

WB, IHC, IF-P, Indirect ELISA

别名

CD276, B7H3, B7 homolog 3, B7 H3, 4Ig-B7-H3

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66481-1-PBS targets B7-H3 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, pig samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 human, pig
免疫原 B7-H3 fusion protein Ag6006 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 CD276 molecule
别名 CD276, B7H3, B7 homolog 3, B7 H3, 4Ig-B7-H3
计算分子量 57 kDa
观测分子量 100 kDa
GenBank蛋白编号BC062581
基因名称 CD276
Gene ID (NCBI) 80381
RRIDAB_2881847
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ5ZPR3
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

B7-H3 (CD276) is a type I transmembrane protein expressed on many tissues and cell types. B7-H3 is a 100-kDa glycoprotein that belongs to the B7 immunoregulatory family and participates in the regulation of T-cell-mediated immune response probably by functioning as both a T cell costimulator and coinhibitor (PMID: 25567370; 20696859). Overexpressed on a wide range of human solid cancers, B7-H3 has been implicated in cancer progression and metastasis and becomes an attractive target for cancer immunotherapy (PMID: 27208063).