• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 66817-1-Ig (same clone as 66817-1-PBS)

    WB analysis using 66817-1-Ig (same clone as 66817-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66817-1-Ig (B7‑H4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66817-1-PBS in a different storage buffer formulation.

  • IHC staining of rat kidney using 66817-1-Ig (same clone as 66817-1-PBS)

    IHC staining of rat kidney using 66817-1-Ig (same clone as 66817-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 66817-1-Ig (B7‑H4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66817-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with VTCN1 antibody included

B7‑H4 Monoclonal antibody, PBS Only

B7‑H4 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66817-1-PBS

产品说明书

CloneNo. 2E5B1

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

VTCN1, B7-H4, B7H4, B7h.5, B7 homolog 4

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy VTCN1 Ready-To-Use IHC Kit. VTCN1 primary antibody included.

产品信息

66817-1-PBS targets B7‑H4 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 B7‑H4 fusion protein Ag27751 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 V-set domain containing T cell activation inhibitor 1
别名 VTCN1, B7-H4, B7H4, B7h.5, B7 homolog 4
计算分子量 282 aa, 31 kDa
观测分子量 31-35 kDa
GenBank蛋白编号BC065717
基因名称 B7-H4
Gene ID (NCBI) 79679
RRIDAB_2882160
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ7Z7D3
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

B7‑H4 also named VTCN1, B7X, or B7S1 is a 282 amino acid protein, which contains 2 immunoglobulin-like domains and belongs to the immunoglobulin superfamily. B7‑H4 negatively regulates T-cell mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. B7‑H4 is a single-pass type I membrane protein, which is over-expressed in breast, ovarian, endometrial, renal cell and non-small-cell lung cancers. The predicted molecular weight of B7‑H4 is 31 kDa. The glycosylated B7‑H4 is 50 to 80 kDa, and the non-glycosylated form is 28 kDa.