• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

B7‑H4 Monoclonal antibody, PBS Only

B7‑H4 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2E5B1

Cat no : 66817-1-PBS

Print datasheet

Synonyms

VTCN1, B7-H4, B7H4, B7h.5, B7 homolog 4

验证数据展示

  • WB analysis using 66817-1-Ig (same clone as 66817-1-PBS)

    WB analysis using 66817-1-Ig (same clone as 66817-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66817-1-Ig (B7‑H4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66817-1-PBS in a different storage buffer formulation.

  • IHC staining of rat kidney using 66817-1-Ig (same clone as 66817-1-PBS)

    IHC staining of rat kidney using 66817-1-Ig (same clone as 66817-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 66817-1-Ig (B7‑H4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66817-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with VTCN1 antibody included
Planning an IHC experiment? We recommend our IHCeasy VTCN1 Ready-To-Use IHC Kit. VTCN1 primary antibody included.

产品信息

66817-1-PBS targets B7‑H4 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen B7‑H4 fusion protein Ag27751 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name V-set domain containing T cell activation inhibitor 1
Synonyms VTCN1, B7-H4, B7H4, B7h.5, B7 homolog 4
Calculated Molecular Weight 282 aa, 31 kDa
Observed Molecular Weight 31-35 kDa
GenBank Accession NumberBC065717
Gene Symbol B7-H4
Gene ID (NCBI) 79679
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ7Z7D3
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

B7‑H4 also named VTCN1, B7X, or B7S1 is a 282 amino acid protein, which contains 2 immunoglobulin-like domains and belongs to the immunoglobulin superfamily. B7‑H4 negatively regulates T-cell mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. B7‑H4 is a single-pass type I membrane protein, which is over-expressed in breast, ovarian, endometrial, renal cell and non-small-cell lung cancers. The predicted molecular weight of B7‑H4 is 31 kDa. The glycosylated B7‑H4 is 50 to 80 kDa, and the non-glycosylated form is 28 kDa.