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B9D2 Polyclonal antibody

B9D2 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IHC, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 22058-1-AP

Print datasheet

Synonyms

B9 domain containing protein 2, B9 protein domain 2, B9D2, ICIS 1

验证数据展示

  • IHC staining of human colon cancer using 22058-1-AP

    IHC staining of human colon cancer using 22058-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22058-1-AP (B9D2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 22058-1-AP

    IHC staining of human colon cancer using 22058-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22058-1-AP (B9D2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22058-1-AP targets B9D2 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human
Cited Reactivityhuman, zebrafish
Immunogen B9D2 fusion protein Ag17104 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name B9 protein domain 2
Synonyms B9 domain containing protein 2, B9 protein domain 2, B9D2, ICIS 1
Calculated Molecular Weight 175 aa, 19 kDa
GenBank Accession NumberBC004444
Gene Symbol B9D2
Gene ID (NCBI) 80776
RRIDAB_2878981
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BPU9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for B9D2 antibody 22058-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,zebrafishWB

Dis Model Mech

Variable phenotypes and penetrance between and within different zebrafish ciliary transition zone mutants

Authors - Jun Wang
View Article
WB,IF

bioRxiv

Two functional forms of the Meckel-Gruber syndrome protein TMEM67 generated by proteolytic cleavage by ADAMTS9 mediate Wnt signaling and ciliogenesis

Authors - Manu Ahmed
View Article