• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

BAALC Polyclonal antibody

BAALC Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 24997-1-AP

Print datasheet

Synonyms

BAALC

验证数据展示

  • WB analysis of HEK-293 using 24997-1-AP

    WB analysis of HEK-293 using 24997-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 24997-1-AP (BAALC Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 24997-1-AP

    IP experiment of mouse brain using 24997-1-AP

    IP result of anti-BAALC (IP:24997-1-AP, 4ug; Detection:24997-1-AP 1:300) with mouse brain tissue lysate 3000ug.

  • IF Staining of HEK-293 using 24997-1-AP

    IF Staining of HEK-293 using 24997-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 24997-1-AP (BAALC antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • IHC staining of mouse brain using 24997-1-AP

    IHC staining of mouse brain using 24997-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24997-1-AP (BAALC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHEK-293 cells, A375 cells, mouse brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

The immunogen of 24997-1-AP is BAALC Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsIHC
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen BAALC fusion protein Ag21850 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name brain and acute leukemia, cytoplasmic
Synonyms BAALC
Calculated Molecular Weight 19 kDa
Observed Molecular Weight 19-25 kDa
GenBank Accession NumberBC011517
Gene Symbol BAALC
Gene ID (NCBI) 79870
RRIDAB_2879837
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WXS3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Brain and acute leukemia cytoplasmic protein, also named as BAALC, is a 180 amino acid protein, which interacts with CAMK2A. BAALC localizes in the cytoplasm and membrane. BAALC is expressed in neural and hematopoietic cells. Expression is found both in normal early progenitor cells and in the most immature type of blasts in acute leukemia but not in mature hematopoietic cells. BAALC may play a synaptic role at the postsynaptic lipid rafts by interacting with CAMK2A.

实验方案

Product Specific Protocols
WB protocol for BAALC antibody 24997-1-APDownload protocol
IHC protocol for BAALC antibody 24997-1-APDownload protocol
IF protocol for BAALC antibody 24997-1-APDownload protocol
IP protocol for BAALC antibody 24997-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Chin Med J (Engl)

Expression and prognostic value of brain and acute leukemia, cytoplasmic in meningiomas.

Authors - Yong-Tao He
View Article