• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of human liver using 15990-1-AP

    WB analysis of human liver using 15990-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 15990-1-AP (BAAT antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human hepatocirrhosis using 15990-1-AP

    IHC staining of human hepatocirrhosis using 15990-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 15990-1-AP (BAAT Antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human hepatocirrhosis using 15990-1-AP

    IHC staining of human hepatocirrhosis using 15990-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 15990-1-AP (BAAT Antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of HepG2 using 15990-1-AP

    IF Staining of HepG2 using 15990-1-AP

    Immunofluorescent analysis of HepG2 cells using 15990-1-AP (BAAT antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

BAAT Polyclonal antibody

BAAT Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 15990-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

BAAT, BACAT, BAT, Glycine N choloyltransferase

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inhuman liver tissue
Positive IHC detected inhuman hepatocirrhosis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15990-1-AP targets BAAT in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 BAAT fusion protein Ag8860 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 bile acid Coenzyme A: amino acid N-acyltransferase (glycine N-choloyltransferase)
别名 BAAT, BACAT, BAT, Glycine N choloyltransferase
计算分子量 418 aa, 46 kDa
观测分子量 50 kDa
GenBank蛋白编号BC009567
基因名称 BAAT
Gene ID (NCBI) 570
RRIDAB_2061361
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ14032
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BAAT is bile acid-CoA:amino acid N-acyltransferase which involved in in bile acid metabolism. BAAT acts as an acyl-CoA thioesterase that regulates intracellular levels of free fatty acids.

实验方案

Product Specific Protocols
WB protocol for BAAT antibody 15990-1-APDownload protocol
IHC protocol for BAAT antibody 15990-1-APDownload protocol
IF protocol for BAAT antibody 15990-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Toxicol In Vitro

Toxicity and intracellular accumulation of bile acids in sandwich-cultured rat hepatocytes: Role of glycine conjugates.

Authors - Chatterjee Sagnik S
View Article