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BACE2 Polyclonal antibody

BACE2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16321-1-AP

Print datasheet

Synonyms

AEPLC, ALP56, Asp 1, ASP1, ASP21, Aspartic like protease 56 kDa, Aspartyl protease 1, BACE2, BAE2, Beta secretase 2, CDA13, CEAP1, Down region aspartic protease, DRAP, Memapsin 1, Theta secretase, UNQ418/PRO852

验证数据展示

  • WB analysis using 16321-1-AP

    WB analysis using 16321-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16321-1-AP (BACE2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 16321-1-AP

    IHC staining of human colon cancer using 16321-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16321-1-AP (BACE2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 16321-1-AP

    IHC staining of human colon cancer using 16321-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16321-1-AP (BACE2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHEK-293 cells, Hela cells, SH-SY5Y cells
Positive IHC detected inhuman colon cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

16321-1-AP targets BACE2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen BACE2 fusion protein Ag9404 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name beta-site APP-cleaving enzyme 2
Synonyms AEPLC, ALP56, Asp 1, ASP1, ASP21, Aspartic like protease 56 kDa, Aspartyl protease 1, BACE2, BAE2, Beta secretase 2, CDA13, CEAP1, Down region aspartic protease, DRAP, Memapsin 1, Theta secretase, UNQ418/PRO852
Calculated Molecular Weight 518 aa, 56 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC014453
Gene Symbol BACE2
Gene ID (NCBI) 25825
RRIDAB_2061667
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y5Z0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for BACE2 antibody 16321-1-APDownload protocol
IHC protocol for BACE2 antibody 16321-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Brain Behav Immun

Elevated β-secretase 1 expression mediates CD4+ T cell dysfunction via PGE2 signalling in Alzheimer's disease.

Authors - Linbin Dai
View Article
mouseWB

Int J Neurosci

Activating transcription factor 6 reduces Aβ1-42 and restores memory in Alzheimer's disease model mice.

Authors - Yayun Du
View Article