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验证数据展示

  • WB analysis using 82842-7-RR

    WB analysis using 82842-7-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82842-7-RR (BACH1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 82842-7-RR

    IF Staining of HeLa using 82842-7-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BACH1 antibody (82842-7-RR, Clone: 4K14 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

BACH1 Recombinant antibody

BACH1 Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 82842-7-RR

产品说明书

CloneNo. 4K14

宿主/亚型

Rabbit / IgG

种属反应性

human, rat, pig

应用

WB, IF/ICC, ELISA

别名

BACH-1, BACH 1, 4K14

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, Jurkat cells, K-562 cells, Raji cells, rat brain tissue, pig brain tissue
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82842-7-RR targets BACH1 in WB, IF/ICC, ELISA applications and shows reactivity with human, rat, pig samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, rat, pig
免疫原 BACH1 fusion protein Ag5128 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 BTB and CNC homology 1, basic leucine zipper transcription factor 1
别名 BACH-1, BACH 1, 4K14
计算分子量 82 kDa
观测分子量100-110 kDa
GenBank蛋白编号BC063307
基因名称 BACH1
Gene ID (NCBI) 571
ENSEMBL Gene IDENSG00000156273
RRIDAB_3086551
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO14867
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BTB and CNC homology 1, basic leucine zipper transcription factor 1(BACH1) is a heme-regulated transcriptional regulator that acts as a repressor or activator and a member of the AP-a superfamily. MafK belongs to the small Maf family that can function as transcriptional activators or repressors depending on the proteins with which they form heterodimers. BACH1 has crucial roles in both stress responses and transformation by H-RasV12. The molecular weight of expected mobility Bach1 is 100-110 kDa.

实验方案

Product Specific Protocols
WB protocol for BACH1 antibody 82842-7-RRDownload protocol
IF protocol for BACH1 antibody 82842-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols