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BAD Monoclonal antibody, PBS Only

BAD Monoclonal Antibody for WB,Indirect ELISA,IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB,Indirect ELISA,IF

Conjugate

Unconjugated

CloneNo.

4B12G2

Cat No : 67830-1-PBS

Print datasheet

Synonyms

BAD, BBC2, BBC6

验证数据展示

  • WB analysis using 67830-1-Ig (same clone as 67830-1-PBS)

    WB analysis using 67830-1-Ig (same clone as 67830-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67830-1-Ig (BAD antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67830-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 67830-1-Ig (same clone as 67830-1-PBS)

    IF Staining of HeLa using 67830-1-Ig (same clone as 67830-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using BAD antibody (67830-1-Ig, Clone: 4B12G2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67830-1-PBS in a different storage buffer formulation.

产品信息

67830-1-PBS targets BAD in WB, IF, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB,Indirect ELISA,IF Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name BCL2-associated agonist of cell death
Synonyms BAD, BBC2, BBC6
Calculated Molecular Weight 18 kDa
Observed Molecular Weight18-25 kDa
GenBank Accession NumberBC001901
Gene Symbol BAD
Gene ID (NCBI) 572
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ92934
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

BAD, also named as BBC6 and BCL2L8, promotes cell death. It is successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. BAD can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2. It appears to act as a link between growth factor receptor signaling and the apoptotic pathways.