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验证数据展示

  • WB analysis using 84541-3-RR

    WB analysis using 84541-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84541-3-RR (BAFF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of U-937 using 84541-3-RR

    WB analysis of U-937 using 84541-3-RR

    U-937 cells were subjected to SDS PAGE followed by western blot with 84541-3-RR (BAFF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

BAFF Recombinant antibody

BAFF Recombinant Antibody for WB, ELISA
Cat No. 84541-3-RR

产品说明书

CloneNo. 242006E4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, ELISA

别名

242006E4

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inU-937 cells, Jurkat cells, RAW 264.7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84541-3-RR targets BAFF in WB, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse
免疫原 FusionProtein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 tumor necrosis factor (ligand) superfamily, member 13b
别名 242006E4
计算分子量34kd
观测分子量 32kd, 17kd
GenBank蛋白编号NM_033622
基因名称 Tnfsf13b
Gene ID (NCBI) 24099
RRIDAB_3672046
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ9WU72
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BAFF (B-cell-activating factor), also called Tnfsf13b (Tumor necrosis factor ligand superfamily member 13B) and CD257, is a cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in stimulating B- and T-cell function and the regulation of humoral immunity. A third B-cell-specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response (PMID: 12867412). BAFF cleaved into 2 chains: membrane form is 32-35 kDa; soluble form is 17 kDa.

实验方案

Product Specific Protocols
WB protocol for BAFF antibody 84541-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols