• 概述
  • 经过测试的应用
  • 产品信息
  • 发表文章 (1)
  • 实验方案
  • Featured Product
  • KD/KO Validated

BAG1S/1M/1L Polyclonal antibody

BAG1S/1M/1L Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 16150-1-AP

Print datasheet

Synonyms

BAG1, BAG 1, HAP, Bcl-2-associated athanogene 1, BAG1L speicfic



经过测试的应用

Positive WB detected inmouse brain tissue, Jurkat cells, mouse liver tissue
Positive IP detected inJurkat cells
Positive IHC detected inhuman testis tissue, human kidney tissue, human lung tissue, human ovary tissue, human placenta tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16150-1-AP targets BAG1S/1M/1L in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IP, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name BCL2-associated athanogene
Synonyms BAG1, BAG 1, HAP, Bcl-2-associated athanogene 1, BAG1L speicfic
Calculated Molecular Weight 26 kDa
Observed Molecular Weight 46-50 kDa, 41-46 kDa, 25-33 kDa
GenBank Accession NumberNM_004323
Gene Symbol BAG1
Gene ID (NCBI) 573
RRIDAB_2062288
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99933
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BAG1 has been identified that modulates gene transcription through poorly defined mechanisms. Four isoforms of the BAG1 protein (BAG1S, BAG1, BAG1M, and BAG1L) can be produced from a common mRNA by use of alternative translation initiation sites, including a non-canonical CTG codon in one instance. The longest, BAG1L (Mr ~50K), contains a nuclear localization signal (NLS) and resides in the nucleus, whereas BAG1M (Mr ~46K) has an incomplete NLS and distributes mainly in cytosol, unless dragged into the nucleus through interactions with other. Distribution of BAG1S(p33) is not clear yet. This antibody recognizes three isoforms of BAG1, includes GAG1L, BAG1M, and BAG1S.

实验方案

Product Specific Protocols
WB protocol for BAG1S/1M/1L antibody 16150-1-APDownload protocol
IHC protocol for BAG1S/1M/1L antibody 16150-1-APDownload protocol
IP protocol for BAG1S/1M/1L antibody 16150-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

  • Journal Impact Factor
  • Most recent
SpeciesApplicationTitle
humanWB,IHC

Tumour Biol

Silencing Bag-1 gene via magnetic gold nanoparticle-delivered siRNA plasmid for colorectal cancer therapy in vivo and in vitro.

Authors - Wenbai Huang
  • KD Validated