- Featured Product
- KD/KO Validated
BAG1S/1M/1L Polyclonal antibody
BAG1S/1M/1L Polyclonal Antibody for WB, IHC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, IP, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | mouse brain tissue, Jurkat cells, mouse liver tissue |
Positive IP detected in | Jurkat cells |
Positive IHC detected in | human testis tissue, human kidney tissue, human lung tissue, human ovary tissue, human placenta tissue, human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
KD/KO | See 1 publications below |
WB | See 1 publications below |
IHC | See 1 publications below |
产品信息
16150-1-AP targets BAG1S/1M/1L in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | WB, IHC, IP, ELISA Application Description |
Cited Applications | WB, IHC |
Tested Reactivity | human, mouse |
Cited Reactivity | human |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | BCL2-associated athanogene |
Synonyms | BAG1, BAG 1, HAP, Bcl-2-associated athanogene 1, BAG1L speicfic |
Calculated Molecular Weight | 26 kDa |
Observed Molecular Weight | 46-50 kDa, 41-46 kDa, 25-33 kDa |
GenBank Accession Number | NM_004323 |
Gene Symbol | BAG1 |
Gene ID (NCBI) | 573 |
RRID | AB_2062288 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q99933 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for BAG1S/1M/1L antibody 16150-1-AP | Download protocol |
IHC protocol for BAG1S/1M/1L antibody 16150-1-AP | Download protocol |
IP protocol for BAG1S/1M/1L antibody 16150-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |