BAG3 Recombinant antibody
BAG3 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, FC (Intra), ELISA
Conjugate
Unconjugated
CloneNo.
240860F12
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HEK-293 cells, K-562 cells, DU 145 cells |
| Positive FC (Intra) detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy BAG3 Ready-To-Use IHC Kit. BAG3 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
The immunogen of 83779-4-RR is BAG3 Fusion Protein expressed in E. coli.
| Tested Applications | WB, FC (Intra), ELISA Application Description |
| Tested Reactivity | human |
| Immunogen | BAG3 fusion protein Ag0956 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | BCL2-associated athanogene 3 |
| Synonyms | Docking protein CAIR-1, BIS, Bcl-2-binding protein Bis, Bcl-2-associated athanogene 3, BAG-3 |
| Calculated Molecular Weight | 61 kDa |
| Observed Molecular Weight | 74-80 kDa |
| GenBank Accession Number | BC006418 |
| Gene Symbol | BAG3 |
| Gene ID (NCBI) | 9531 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | O95817 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
BAG3 (Bcl2-associated athanogene 3) belongs to the BAG protein family, the co-chaperone that binds to Hsc70/Hsp70 through the BAG domain and modulates their activity in polypeptide folding. BAG3 contains also a WW domain and a proline-rich (PXXP) repeat, that mediate binding to partners different from Hsp70. Through interacting with different molecular partner, BAG3 influences several cell processes, such as apoptosis, autophagy and cell motility. BAG3 protein has been reported to sustain cell survival, resistance to therapy, and/or motility and metastatization in several tumor types, thus being identified as a potential target for anticancer therapies. In addition, defects in BAG3 are the cause of some myopathy. BAG3 normally migrates around 74-80 kDa; a slightly different molecular weight or a doublet form can be observed in some cell types and/or following cell exposure to stressors. A synaptosome associated form of 40 kDa has recently been described.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for BAG3 antibody 83779-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |