• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of K-562 using 13913-1-AP

    WB analysis of K-562 using 13913-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 13913-1-AP (SODD/BAG4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of K-562 using 13913-1-AP

    IP experiment of K-562 using 13913-1-AP

    IP result of anti-SODD/BAG4 (IP:13913-1-AP, 4ug; Detection:13913-1-AP 1:500) with K-562 cells lysate 2800ug.

  • IF Staining of MCF-7 using 13913-1-AP

    IF Staining of MCF-7 using 13913-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using SODD/BAG4 antibody (13913-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

SODD/BAG4 Polyclonal antibody

SODD/BAG4 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 13913-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IF/ICC, IP, ELISA

别名

SODD, BAG4, BAG 4, Silencer of death domains, Bcl-2-associated athanogene 4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inK-562 cells, MCF-7 cells
Positive IP detected inK-562 cells
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

13913-1-AP targets SODD/BAG4 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性artemia sinica
免疫原 SODD/BAG4 fusion protein Ag4905 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 BCL2-associated athanogene 4
别名 SODD, BAG4, BAG 4, Silencer of death domains, Bcl-2-associated athanogene 4
计算分子量 457 aa, 50 kDa
观测分子量 60 kDa, 66-70 kDa
GenBank蛋白编号BC038505
基因名称 SODD/BAG4
Gene ID (NCBI) 9530
RRIDAB_2243308
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO95429
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BAG4, also known as SODD (the silencer of death domain), belongs to the BAG family of anti-apoptotic proteins. BAG4 binds to the tumor necrosis factor receptor 1 (TNFR1) and block signaling for subsequent activation of apoptosis. This antibody detects 66-70 kDa full-length BAG4 protein and 45 kDa, 60 kDa C-terminal fragments, which was also observed by Hedda et al. (12706861).

实验方案

Product Specific Protocols
WB protocol for SODD/BAG4 antibody 13913-1-APDownload protocol
IF protocol for SODD/BAG4 antibody 13913-1-APDownload protocol
IP protocol for SODD/BAG4 antibody 13913-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
Artemia sinicaWB

Gene

Identification, expression pattern and functional characterization of As-kip2 in diapause embryo restarting process of Artemia sinica.

Authors - Mengchen Zhang
View Article
WB

Nat Commun

Mettl3-dependent m6A modification attenuates the brain stress response in Drosophila

Authors - Alexandra E. Perlegos
View Article