验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, Neuro-2a cells, PC-12 cells, HeLa cells, THP-1 cells |
Positive IP detected in | HEK-293 cells |
Positive IF/ICC detected in | HeLa cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 1 publications below |
产品信息
30017-1-AP targets BAK in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IF/ICC, IP, ELISA Application Description |
Cited Applications | WB |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | duck |
Immunogen | BAK fusion protein Ag27958 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | BCL2-antagonist/killer 1 |
Synonyms | BAK1, CDN1, Bcl-2-like protein 7, Bcl2-L-7, Bcl-2 homologous antagonist/killer |
Calculated Molecular Weight | 23 kDa |
Observed Molecular Weight | 23-25 kDa |
GenBank Accession Number | BC004431 |
Gene Symbol | BAK1 |
Gene ID (NCBI) | 578 |
RRID | AB_3086211 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q16611 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form oligomers or heterodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome c. This protein also interacts with the tumor suppressor P53 after exposure to cell stress.
实验方案
Product Specific Protocols | |
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WB protocol for BAK antibody 30017-1-AP | Download protocol |
IF protocol for BAK antibody 30017-1-AP | Download protocol |
IP protocol for BAK antibody 30017-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |