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BASP1 Polyclonal antibody

BASP1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14880-1-AP

Print datasheet

Synonyms

NAP22, NAP 22, CAP23, CAP 23, Brain acid soluble protein 1

验证数据展示

  • WB analysis using 14880-1-AP

    WB analysis using 14880-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14880-1-AP (BASP1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IHC staining of human skin cancer using 14880-1-AP

    IHC staining of human skin cancer using 14880-1-AP

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 14880-1-AP (BASP1 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with BASP1 antibody included
  • IHC staining of human skin cancer using 14880-1-AP

    IHC staining of human skin cancer using 14880-1-AP

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 14880-1-AP (BASP1 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with BASP1 antibody included

经过测试的应用

Positive WB detected inDU 145 cells, A549 cells, HeLa cells, fetal human brain tissue
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy BASP1 Ready-To-Use IHC Kit. BASP1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:800-1:3200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14880-1-AP targets BASP1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen BASP1 fusion protein Ag6653 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name brain abundant, membrane attached signal protein 1
Synonyms NAP22, NAP 22, CAP23, CAP 23, Brain acid soluble protein 1
Calculated Molecular Weight 23 kDa
Observed Molecular Weight 55-60 kDa
GenBank Accession NumberBC000518
Gene Symbol BASP1
Gene ID (NCBI) 10409
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP80723
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for BASP1 antibody 14880-1-APDownload protocol
IHC protocol for BASP1 antibody 14880-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols