验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, rat spleen tissue |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy DDX39B Ready-To-Use IHC Kit. DDX39B primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14798-1-AP targets DDX39B in WB, IHC, IF, IP, CoIP, ChIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP, ChIP, RIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, pig, chicken |
| 免疫原 | DDX39B fusion protein Ag6512 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | HLA-B associated transcript 1 |
| 别名 | ATP dependent RNA helicase p47, BAT1, D6S81E, DDX39, DDX39B, DEAD box protein UAP56, HLA B associated transcript 1, Spliceosome RNA helicase BAT1, UAP56 |
| 计算分子量 | 53 kDa |
| 观测分子量 | 48 kDa |
| GenBank蛋白编号 | BC000361 |
| 基因名称 | BAT1 |
| Gene ID (NCBI) | 7919 |
| RRID | AB_2061854 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q13838 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DDX39B, is a 428 amino acid protein, which contains one helicase C-terminal domain, one helicase ATP-binding domain and belongs to the DEAD box helicase family. DECD subfamily. DDX39B localizes in the nucleus and cytoplasm. BAT1 is involved in nuclear export of spliced and unspliced mRNA. BAT1 as ATP dependent RNA helicase, may be a translation initiation factor and acts as a negative regulator of inflammatory cytokines. This antibody may cross-react with DDX39A.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DDX39B antibody 14798-1-AP | Download protocol |
| IHC protocol for DDX39B antibody 14798-1-AP | Download protocol |
| IP protocol for DDX39B antibody 14798-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Metab NEAT1 is essential for metabolic changes that promote breast cancer growth and metastasis. | ||
Genes Dev UAP56/DDX39B is a major cotranscriptional RNA-DNA helicase that unwinds harmful R loops genome-wide. | ||
Cell Death Discov DDX39B contributes to the proliferation of colorectal cancer through direct binding to CDK6/CCND1.
| ||
iScience THOC5 complexes with DDX5, DDX17, and CDK12 to regulate R loop structures and transcription elongation rate | ||
ACS Infect Dis Porcine ZC3H11A Is Essential for the Proliferation of Pseudorabies Virus and Porcine Circovirus 2. | ||
Sci Rep ALS Associated Mutations in Matrin 3 Alter Protein-Protein Interactions and Impede mRNA Nuclear Export. |
