验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, rat spleen tissue |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy DDX39B Ready-To-Use IHC Kit. DDX39B primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14798-1-AP targets DDX39B in WB, IHC, IF, IP, CoIP, ChIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IP, IHC, ELISA Application Description |
| Cited Applications | WB, IHC, IF, IP, CoIP, ChIP, RIP |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, pig, chicken |
| Immunogen | DDX39B fusion protein Ag6512 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | HLA-B associated transcript 1 |
| Synonyms | ATP dependent RNA helicase p47, BAT1, D6S81E, DDX39, DDX39B, DEAD box protein UAP56, HLA B associated transcript 1, Spliceosome RNA helicase BAT1, UAP56 |
| Calculated Molecular Weight | 53 kDa |
| Observed Molecular Weight | 48 kDa |
| GenBank Accession Number | BC000361 |
| Gene Symbol | BAT1 |
| Gene ID (NCBI) | 7919 |
| RRID | AB_2061854 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13838 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DDX39B, is a 428 amino acid protein, which contains one helicase C-terminal domain, one helicase ATP-binding domain and belongs to the DEAD box helicase family. DECD subfamily. DDX39B localizes in the nucleus and cytoplasm. BAT1 is involved in nuclear export of spliced and unspliced mRNA. BAT1 as ATP dependent RNA helicase, may be a translation initiation factor and acts as a negative regulator of inflammatory cytokines. This antibody may cross-react with DDX39A.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DDX39B antibody 14798-1-AP | Download protocol |
| IHC protocol for DDX39B antibody 14798-1-AP | Download protocol |
| IP protocol for DDX39B antibody 14798-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Metab NEAT1 is essential for metabolic changes that promote breast cancer growth and metastasis. | ||
Genes Dev UAP56/DDX39B is a major cotranscriptional RNA-DNA helicase that unwinds harmful R loops genome-wide. | ||
Cell Death Discov DDX39B contributes to the proliferation of colorectal cancer through direct binding to CDK6/CCND1.
| ||
iScience THOC5 complexes with DDX5, DDX17, and CDK12 to regulate R loop structures and transcription elongation rate | ||
ACS Infect Dis Porcine ZC3H11A Is Essential for the Proliferation of Pseudorabies Virus and Porcine Circovirus 2. | ||
Sci Rep ALS Associated Mutations in Matrin 3 Alter Protein-Protein Interactions and Impede mRNA Nuclear Export. |
