验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, NIH/3T3 cells, 4T1 cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
67641-1-Ig targets BAT1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, IP, ELISA Application Description |
经测试反应性 | human, mouse, rat |
免疫原 | BAT1 fusion protein Ag6612 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | HLA-B associated transcript 1 |
别名 | DDX39B, UAP56, DDX39, D6S81E, ATP-dependent RNA helicase p47 |
计算分子量 | 53 kDa |
观测分子量 | 50 kDa |
GenBank蛋白编号 | BC000361 |
基因名称 | BAT1 |
Gene ID (NCBI) | 7919 |
RRID | AB_2882841 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein G purification |
UNIPROT ID | Q13838 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DDX39B, is a 428 amino acid protein, which contains one helicase C-terminal domain, one helicase ATP-binding domain and belongs to the DEAD box helicase family. DECD subfamily. DDX39B localizes in the nucleus and cytoplasm. BAT1 is involved in nuclear export of spliced and unspliced mRNA. BAT1 as ATP dependent RNA helicase, may be a translation initiation factor and acts as a negative regulator of inflammatory cytokines. This antibody may cross-react with DDX39A.
实验方案
Product Specific Protocols | |
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WB protocol for BAT1 antibody 67641-1-Ig | Download protocol |
IHC protocol for BAT1 antibody 67641-1-Ig | Download protocol |
IP protocol for BAT1 antibody 67641-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |