验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, NIH/3T3 cells, 4T1 cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
67641-1-Ig targets BAT1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, IP, ELISA Application Description |
Tested Reactivity | human, mouse, rat |
Immunogen | BAT1 fusion protein Ag6612 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | HLA-B associated transcript 1 |
Synonyms | DDX39B, UAP56, DDX39, D6S81E, ATP-dependent RNA helicase p47 |
Calculated Molecular Weight | 53 kDa |
Observed Molecular Weight | 50 kDa |
GenBank Accession Number | BC000361 |
Gene Symbol | BAT1 |
Gene ID (NCBI) | 7919 |
RRID | AB_2882841 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | Q13838 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DDX39B, is a 428 amino acid protein, which contains one helicase C-terminal domain, one helicase ATP-binding domain and belongs to the DEAD box helicase family. DECD subfamily. DDX39B localizes in the nucleus and cytoplasm. BAT1 is involved in nuclear export of spliced and unspliced mRNA. BAT1 as ATP dependent RNA helicase, may be a translation initiation factor and acts as a negative regulator of inflammatory cytokines. This antibody may cross-react with DDX39A.
实验方案
Product Specific Protocols | |
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WB protocol for BAT1 antibody 67641-1-Ig | Download protocol |
IHC protocol for BAT1 antibody 67641-1-Ig | Download protocol |
IP protocol for BAT1 antibody 67641-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |