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验证数据展示

  • WB analysis using 30984-1-AP

    WB analysis using 30984-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30984-1-AP (BAZ2A antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

BAZ2A Polyclonal antibody

BAZ2A Polyclonal Antibody for WB, ELISA
Cat No. 30984-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse

应用

WB, ELISA

别名

BAZ2A, DKFZp781B109, FLJ13768, FLJ13780, FLJ45876, hWALp3, KIAA0314, TIP5, TTF I interacting protein 5, WALp3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293T cells, HeLa cells, PC-3 cells, NIH/3T3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30984-1-AP targets BAZ2A in WB, ELISA applications and shows reactivity with Human, mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human, mouse
免疫原 BAZ2A fusion protein Ag33963 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 bromodomain adjacent to zinc finger domain, 2A
别名 BAZ2A, DKFZp781B109, FLJ13768, FLJ13780, FLJ45876, hWALp3, KIAA0314, TIP5, TTF I interacting protein 5, WALp3
计算分子量211 kDa
观测分子量250-270 kDa
GenBank蛋白编号NM_013449.4
基因名称 BAZ2A
Gene ID (NCBI) 11176
RRIDAB_3669805
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ9UIF9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BAZ2A (bromodomain adjacent to zinc finger domain 2A), also known as TIP5. It is expected to be located in nucleus, and the protein is expressed at moderate levels in most tissues analyzed, including heart, brain, placenta, lung, skeletal muscle, kidney and pancreas. BAZ2A is an epigenetic regulator affecting transcription of ribosomal RNA, it is also a regulatory subunit of the ATP-dependent NoRC-1 and NoRC-5 ISWI chromatin remodeling complexes, which form ordered nucleosome arrays on chromatin and facilitate access to DNA during DNA-templated processes such as DNA replication, transcription, and repair (PubMed:28801535). The calculated molecular weight of BAZ2A is 210 kDa.

实验方案

Product Specific Protocols
WB protocol for BAZ2A antibody 30984-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols